2X2Q

Crystal structure of an 'all locked' LNA duplex at 1.9 angstrom resolution

Summary for 2X2Q

• Entry DOI: 10.2210/pdb2x2q/pdb
• Descriptor: LOCKED NUCLEIC ACID DERIVED FROM TRNA SER ACCEPTOR STEM MICROHELIX, COBALT HEXAMMINE(III), MAGNESIUM ION, ... (6 entities in total)
• Functional Keywords: dna, modified nucleic acid, locked nucleic acid, thermostability
• Biological source: SYNTHETIC CONSTRUCT; More
• Total number of polymer chains: 4
• Total formula weight: 9720.61

Authors

Eichert, A.,Behling, K.,Fuerste, J.P.,Betzel, C.,Erdmann, V.A.,Foerster, C. (deposition date: 2010-01-15, release date: 2011-02-16, Last modification date: 2023-12-20)

Primary citation

Eichert, A.,Behling, K.,Betzel, C.,Erdmann, V.A.,Furste, J.P.,Forster, C.
The Crystal Structure of an 'All Locked' Nucleic Acid Duplex.
Nucleic Acids Res., 38:6729-, 2010

PubMed Abstract: 'Locked nucleic acids' (LNAs) are known to introduce enhanced bio- and thermostability into natural nucleic acids rendering them powerful tools for diagnostic and therapeutic applications. We present the 1.9 Å X-ray structure of an 'all LNA' duplex containing exclusively modified β-D-2'-O-4'C-methylene ribofuranose nucleotides. The helix illustrates a new type of nucleic acid geometry that contributes to the understanding of the enhanced thermostability of LNA duplexes. A notable decrease of several local and overall helical parameters like twist, roll and propeller twist influence the structure of the LNA helix and result in a widening of the major groove, a decrease in helical winding and an enlarged helical pitch. A detailed structural comparison to the previously solved RNA crystal structure with the corresponding base pair sequence underlines the differences in conformation. The surrounding water network of the RNA and the LNA helix shows a similar hydration pattern.

PubMed: 20530536
DOI: 10.1093/NAR/GKQ505

Experimental method

X-RAY DIFFRACTION (1.9 Å)