2X2Q

Crystal structure of an 'all locked' LNA duplex at 1.9 angstrom resolution

2X2Q の概要

• エントリーDOI: 10.2210/pdb2x2q/pdb
• 分子名称: LOCKED NUCLEIC ACID DERIVED FROM TRNA SER ACCEPTOR STEM MICROHELIX, COBALT HEXAMMINE(III), MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: dna, modified nucleic acid, locked nucleic acid, thermostability
• 由来する生物種: SYNTHETIC CONSTRUCT; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 9720.61

構造登録者

Eichert, A.,Behling, K.,Fuerste, J.P.,Betzel, C.,Erdmann, V.A.,Foerster, C. (登録日: 2010-01-15, 公開日: 2011-02-16, 最終更新日: 2023-12-20)

主引用文献

Eichert, A.,Behling, K.,Betzel, C.,Erdmann, V.A.,Furste, J.P.,Forster, C.
The Crystal Structure of an 'All Locked' Nucleic Acid Duplex.
Nucleic Acids Res., 38:6729-, 2010

PubMed Abstract: 'Locked nucleic acids' (LNAs) are known to introduce enhanced bio- and thermostability into natural nucleic acids rendering them powerful tools for diagnostic and therapeutic applications. We present the 1.9 Å X-ray structure of an 'all LNA' duplex containing exclusively modified β-D-2'-O-4'C-methylene ribofuranose nucleotides. The helix illustrates a new type of nucleic acid geometry that contributes to the understanding of the enhanced thermostability of LNA duplexes. A notable decrease of several local and overall helical parameters like twist, roll and propeller twist influence the structure of the LNA helix and result in a widening of the major groove, a decrease in helical winding and an enlarged helical pitch. A detailed structural comparison to the previously solved RNA crystal structure with the corresponding base pair sequence underlines the differences in conformation. The surrounding water network of the RNA and the LNA helix shows a similar hydration pattern.

PubMed: 20530536
DOI: 10.1093/NAR/GKQ505

実験手法

X-RAY DIFFRACTION (1.9 Å)