2WZL

The Structure of the N-RNA Binding Domain of the Mokola virus Phosphoprotein

2WZL の概要

• エントリーDOI: 10.2210/pdb2wzl/pdb
• 分子名称: PHOSPHOPROTEIN, GLYCEROL (3 entities in total)
• 機能のキーワード: viral protein, rabies, virion, chaperone, nucleoprotein, rna replication
• 由来する生物種: MOKOLA VIRUS
• 細胞内の位置: Phosphoprotein: Virion. Isoform P2: Host cytoplasm (By similarity). Isoform P3: Host nucleus (By similarity): P0C569
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34343.12

構造登録者

Assenberg, R.,Delmas, O.,Ren, J.,Vidalain, P.,Verma, A.,Larrous, F.,Graham, S.,Tangy, F.,Grimes, J.,Bourhy, H. (登録日: 2009-11-30, 公開日: 2009-12-15, 最終更新日: 2023-12-20)

主引用文献

Assenberg, R.,Delmas, O.,Ren, J.,Vidalain, P.,Verma, A.,Larrous, F.,Graham, S.,Tangy, F.,Grimes, J.,Bourhy, H.
The Structure of the N-RNA Binding Domain of the Mokola Virus Phosphoprotein
J.Virol., 84:1089-, 2010

PubMed Abstract: Mokola virus (MOKV) is a nonsegmented, negative-sense RNA virus that belongs to the Lyssavirus genus and Rhabdoviridae family. MOKV phosphoprotein P is an essential component of the replication and transcription complex and acts as a cofactor for the viral RNA-dependent RNA polymerase. P recruits the viral polymerase to the nucleoprotein-bound viral RNA (N-RNA) via an interaction between its C-terminal domain and the N-RNA complex. Here we present a structure for this domain of MOKV P, obtained by expression of full-length P in Escherichia coli, which was subsequently truncated during crystallization. The structure has a high degree of homology with P of rabies virus, another member of Lyssavirus genus, and to a lesser degree with P of vesicular stomatitis virus (VSV), a member of the related Vesiculovirus genus. In addition, analysis of the crystal packing of this domain reveals a potential binding site for the nucleoprotein N. Using both site-directed mutagenesis and yeast two-hybrid experiments to measure P-N interaction, we have determined the relative roles of key amino acids involved in this interaction to map the region of P that binds N. This analysis also reveals a structural relationship between the N-RNA binding domain of the P proteins of the Rhabdoviridae and the Paramyxoviridae.

PubMed: 19906936
DOI: 10.1128/JVI.01520-09

実験手法

X-RAY DIFFRACTION (2.1 Å)