2WVP

Synthetically modified OmpG

2WVP の概要

• エントリーDOI: 10.2210/pdb2wvp/pdb
• 関連するPDBエントリー: 2F1C 2IWV 2IWW
• 分子名称: OUTER MEMBRANE PROTEIN G, N-[2-({[5-(DIMETHYLAMINO)NAPHTHALEN-1-YL]SULFONYL}AMINO)ETHYL]-2-IODOACETAMIDE, (HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE, ... (5 entities in total)
• 機能のキーワード: ion-channel engineering, ompg hybrids, membrane protein
• 由来する生物種: ESCHERICHIA COLI
• 細胞内の位置: Cell outer membrane; Multi-pass membrane protein: P76045
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35936.19

構造登録者

Grosse, W.,Reiss, P.,Reitz, S.,Cebi, M.,Luebben, W.,Koert, U.,Essen, L.-O. (登録日: 2009-10-19, 公開日: 2010-07-14, 最終更新日: 2024-11-13)

主引用文献

Grosse, W.,Reiss, P.,Reitz, S.,Cebi, M.,Luebben, W.,Koert, U.,Essen, L.-O.
Structural and Functional Characterization of a Synthetically Modified Ompg.
Bioorg.Med.Chem., 18:7716-, 2010

PubMed Abstract: Chemical modification of ion channels has recently attracted attention due to their potential use in stochastic sensing and neurobiology. Among the available channel templates stable β-barrel proteins have shown their potential for large scale chemical modifications due to their wide pore lumen. Ion-channel hybrids using the outer membrane protein OmpG were generated by S-alkylation with a synthetic modulator and functionally as well as structurally characterized. The dansyl moiety of the used modulator resulted in partial blockage of current though the OmpG channel with its gating characteristics mainly unaffected. The crystal structure of an OmpG-dansyl hybrid at 2.4Å resolution correlates this finding by showing that the modulator lines the inner walling of the OmpG pore. These results underline the suitability of OmpG as a structural base for the construction of stochastic sensors.

PubMed: 20378361
DOI: 10.1016/J.BMC.2010.03.044

実験手法

X-RAY DIFFRACTION (2.4 Å)