2WUX

the crystal structure of recombinant baculovirus polyhedra

Summary for 2WUX

• Entry DOI: 10.2210/pdb2wux/pdb
• Related: 2WUY
• Descriptor: POLYHEDRIN (2 entities in total)
• Functional Keywords: microcrystals, polyhedra, viral protein, viral occlusion body, viral capsid
• Biological source: AUTOGRAPHA CALIFORNICA NUCLEAR POLYHEDROSIS VIRUS
• Total number of polymer chains: 1
• Total formula weight: 28736.76

Authors

Ji, X.,Sutton, G.,Evans, G.,Axford, D.,Owen, R.,Stuart, D.I. (deposition date: 2009-10-10, release date: 2009-12-15, Last modification date: 2011-07-13)

Primary citation

Ji, X.,Sutton, G.,Evans, G.,Axford, D.,Owen, R.,Stuart, D.I.
How Baculovirus Polyhedra Fit Square Pegs Into Round Holes to Robustly Package Viruses.
Embo J., 29:505-, 2010

PubMed Abstract: Natural protein crystals (polyhedra) armour certain viruses, allowing them to survive for years under hostile conditions. We have determined the structure of polyhedra of the baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV), revealing a highly symmetrical covalently cross-braced robust lattice, the subunits of which possess a flexible adaptor enabling this supra-molecular assembly to specifically entrap massive baculoviruses. Inter-subunit chemical switches modulate the controlled release of virus particles in the unusual high pH environment of the target insect's gut. Surprisingly, the polyhedrin subunits are more similar to picornavirus coat proteins than to the polyhedrin of cytoplasmic polyhedrosis virus (CPV). It is, therefore, remarkable that both AcMNPV and CPV polyhedra possess identical crystal lattices and crystal symmetry. This crystalline arrangement must be particularly well suited to the functional requirements of the polyhedra and has been either preserved or re-selected during evolution. The use of flexible adaptors to generate a powerful system for packaging irregular particles is characteristic of the AcMNPV polyhedrin and may provide a vehicle to sequester a wide range of objects such as biological nano-particles.

PubMed: 19959989
DOI: 10.1038/EMBOJ.2009.352

Experimental method

X-RAY DIFFRACTION (1.838 Å)