2WRB

the structure of influenza H2 human singapore hemagglutinin with avian receptor

2WRB の概要

• エントリーDOI: 10.2210/pdb2wrb/pdb
• 関連するPDBエントリー: 2WR0 2WR1 2WR2 2WR3 2WR4 2WR5 2WR7 2WRC 2WRD 2WRE 2WRF 2WRG 2WRH
• 分子名称: HEMAGGLUTININ, N-acetyl-alpha-neuraminic acid (2 entities in total)
• 機能のキーワード: glycoprotein, lipoprotein, envelope protein, viral protein
• 由来する生物種: INFLUENZA A VIRUS
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 171801.98

構造登録者

Liu, J.,Stevens, D.J.,Haire, L.F.,Walker, P.A.,Coombs, P.J.,Russell, R.J.,Gamblin, S.J.,Skehel, J.J. (登録日: 2009-09-01, 公開日: 2009-09-29, 最終更新日: 2024-10-16)

主引用文献

Liu, J.,Stevens, D.J.,Haire, L.F.,Walker, P.A.,Coombs, P.J.,Russell, R.J.,Gamblin, S.J.,Skehel, J.J.
From the Cover: Structures of Receptor Complexes Formed by Hemagglutinins from the Asian Influenza Pandemic of 1957.
Proc.Natl.Acad.Sci.USA, 106:17175-, 2009

PubMed Abstract: The viruses that caused the three influenza pandemics of the twentieth century in 1918, 1957, and 1968 had distinct hemagglutinin receptor binding glycoproteins that had evolved the capacity to recognize human cell receptors. We have determined the structure of the H2 hemagglutinin from the second pandemic, the "Asian Influenza" of 1957. We compare it with the 1918 "Spanish Influenza" hemagglutinin, H1, and the 1968 "Hong Kong Influenza" hemagglutinin, H3, and show that despite its close overall structural similarity to H1, and its more distant relationship to H3, the H2 receptor binding site is closely related to that of H3 hemagglutinin. By analyzing hemagglutinins of potential H2 avian precursors of the pandemic virus, we show that the human receptor can be bound by avian hemagglutinins that lack the human-specific mutations of H2 and H3 pandemic viruses, Gln-226Leu, and Gly-228Ser. We show how Gln-226 in the avian H2 receptor binding site, together with Asn-186, form hydrogen bond networks through bound water molecules to mediate binding to human receptor. We show that the human receptor adopts a very similar conformation in both human and avian hemagglutinin-receptor complexes. We also show that Leu-226 in the receptor binding site of human virus hemagglutinins creates a hydrophobic environment near the Sia-1-Gal-2 glycosidic linkage that favors binding of the human receptor and is unfavorable for avian receptor binding. We consider the significance for the development of pandemics, of the existence of avian viruses that can bind to both avian and human receptors.

PubMed: 19805083
DOI: 10.1073/PNAS.0906849106

実験手法

X-RAY DIFFRACTION (3.1 Å)