2WQK

Crystal Structure of Sure Protein from Aquifex aeolicus

「2PHJ」から置き換えられました

2WQK の概要

• エントリーDOI: 10.2210/pdb2wqk/pdb
• 分子名称: 5'-NUCLEOTIDASE SURE, SULFATE ION, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: sure protein, putative acid phosphatase, structural genomics, 3-d structure, mixed alpha/beta protein, nppsfa, national project on protein structural and functional analyses, riken structural genomics/proteomics initiative, rsgi, hydrolase
• 由来する生物種: AQUIFEX AEOLICUS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 56632.58

構造登録者

Antonyuk, S.V.,Ellis, M.J.,Strange, R.W.,Hasnain, S.S.,Bessho, Y.,Kuramitsu, S.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2009-08-23, 公開日: 2009-09-29, 最終更新日: 2023-12-20)

主引用文献

Antonyuk, S.V.,Ellis, M.J.,Strange, R.W.,Bessho, Y.,Kuramitsu, S.,Shinkai, A.,Yokoyama, S.,Hasnain, S.S.
Structure of Sure Protein from Aquifex Aeolicus Vf5 at 1.5 A Resolution.
Acta Crystallogr.,Sect.F, 65:1204-, 2009

PubMed Abstract: SurE is a stationary-phase survival protein found in bacteria, eukaryotes and archaea that exhibits a divalent-metal-ion-dependent phosphatase activity and acts as a nucleotidase and polyphosphate phosphohydrolase. The structure of the SurE protein from the hyperthermophile Aquifex aeolicus has been solved at 1.5 A resolution using molecular replacement with one dimer in the asymmetric unit and refined to an R factor of 15.6%. The crystal packing reveals that two dimers assemble to form a tetramer, although gel-filtration chromatography showed the presence of only a dimer in solution. The phosphatase active-site pocket was occupied by sulfate ions from the crystallization medium.

PubMed: 20054112
DOI: 10.1107/S1744309109043814

実験手法

X-RAY DIFFRACTION (1.5 Å)