2WNW
The crystal structure of SrfJ from salmonella typhimurium
Summary for 2WNW
| Entry DOI | 10.2210/pdb2wnw/pdb |
| Descriptor | ACTIVATED BY TRANSCRIPTION FACTOR SSRB, PHOSPHATE ION, GLYCEROL, ... (4 entities in total) |
| Functional Keywords | hydrolase, salmonella typhimurium, o-glycosyl hydrolase family 30 |
| Biological source | SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHIMURIUM STR. LT2 |
| Total number of polymer chains | 2 |
| Total formula weight | 102592.56 |
| Authors | Kim, Y.-G.,Kim, J.-H.,Kim, K.-J. (deposition date: 2009-07-20, release date: 2010-03-02, Last modification date: 2024-05-08) |
| Primary citation | Kim, Y.-G.,Kim, J.-H.,Kim, K.-J. Crystal Structure of the Salmonella Enterica Serovar Typhimurium Virulence Factor Srfj, a Glycoside Hydrolase Family Enzyme. J.Bacteriol., 191:6550-, 2009 Cited by PubMed Abstract: To cause infection, Salmonella enterica serovar Typhimurium uses type III secretion systems, which are encoded on two Salmonella pathogenicity islands, SPI-1 and SPI-2, the latter of which is thought to play a crucial role in bacterial proliferation in Salmonella-containing vacuoles (SCVs) after invading cells. S. Typhimurium SrfJ, located outside SPI-2, is also known to be involved in Salmonella pathogenicity and has high amino acid sequence homology with human lysosomal glucosylceramidase (GlcCerase). We present the first crystal structure of SrfJ at a resolution of 1.8 A. The overall fold of SrfJ shares high structure similarities with that of human GlcCerase, comprising two distinctive domains: a (beta/alpha)(8)-barrel catalytic domain and a beta-sandwich domain. As in human GlcCerase, the pocket-shaped active site of SrfJ is located on the C-terminal side of the barrel, and two conserved glutamic acid residues are used for the enzyme catalysis. Moreover, a glycerol-bound form of SrfJ reveals that the glucose ring moiety of the substrate might similarly bind to the enzyme as to human GlcCerase, suggesting that SrfJ might function as a glycoside hydrolase. Although some structural differences are observed between SrfJ and human GlcCerase in the substrate entrance of the active site, we speculate that, based on the high structural similarities to human GlcCerase in the overall fold and the active-site environment, SrfJ might have a GlcCerase activity and use the activity to enhance Salmonella virulence by modifying SCV membrane lipids. PubMed: 19717598DOI: 10.1128/JB.00641-09 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
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