2WLW

Structure of the N-terminal capsid domain of HIV-2

2WLW の概要

• エントリーDOI: 10.2210/pdb2wlw/pdb
• 関連するPDBエントリー: 2WLV
• 分子名称: PEPTIDYL-PROLYL CIS-TRANS ISOMERASE (2 entities in total)
• 機能のキーワード: rotamase, isomerase, rhesus macaque trim-cyp
• 由来する生物種: MACACA MULATTA
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18016.47

構造登録者

Price, A.J.,Marzetta, F.,Lammers, M.,Ylinen, L.M.J.,Schaller, T.,Wilson, S.J.,Towers, G.J.,James, L.C. (登録日: 2009-06-26, 公開日: 2009-09-22, 最終更新日: 2024-05-08)

主引用文献

Price, A.J.,Marzetta, F.,Lammers, M.,Ylinen, L.M.J.,Schaller, T.,Wilson, S.J.,Towers, G.J.,James, L.C.
Active Site Remodelling Switches HIV Specificity of Antiretroviral Trimcyp
Nat.Struct.Mol.Biol., 16:1036-, 2009

PubMed Abstract: TRIMCyps are primate antiretroviral proteins that potently inhibit HIV replication. Here we describe how rhesus macaque TRIMCyp (RhTC) has evolved to target and restrict HIV-2. We show that the ancestral cyclophilin A (CypA) domain of RhTC targets HIV-2 capsid with weak affinity, which is strongly increased in RhTC by two mutations (D66N and R69H) at the expense of HIV-1 binding. These mutations disrupt a constraining intramolecular interaction in CypA, triggering the complete restructuring (>16 A) of an active site loop. This new configuration discriminates between divergent HIV-1 and HIV-2 loop conformations mediated by capsid residue 88. Viral sensitivity to RhTC restriction can be conferred or abolished by mutating position 88. Furthermore, position 88 determines the susceptibility of naturally occurring HIV-1 sequences to restriction. Our results reveal the complex molecular, structural and thermodynamic changes that underlie the ongoing evolutionary race between virus and host.

PubMed: 19767750
DOI: 10.1038/NSMB.1667

実験手法

X-RAY DIFFRACTION (1.5 Å)