2WKW

Alcaligenes esterase complexed with product analogue

2WKW の概要

• エントリーDOI: 10.2210/pdb2wkw/pdb
• 関連するPDBエントリー: 1QLW
• 分子名称: CARBOXYLESTERASE, [(2S)-4-methyl-3-oxo-2,3,4,5-tetrahydro-1H-1,4-benzodiazepin-2-yl]acetic acid, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: hydrolase, hydrolase(carboxylic esterase), esterase, atomic resolution, alpha/beta hydrolase
• 由来する生物種: ALCALIGENES SP.
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 73159.14

構造登録者

Bourne, P.C.,Isupov, M.N.,Littlechild, J.A. (登録日: 2009-06-18, 公開日: 2009-06-30, 最終更新日: 2024-10-16)

主引用文献

Bourne, P.C.,Isupov, M.N.,Littlechild, J.A.
The Atomic-Resolution Structure of a Novel Bacterial Esterase.
Structure, 8:143-, 2000

PubMed Abstract: A novel bacterial esterase that cleaves esters on halogenated cyclic compounds has been isolated from an Alcaligenes species. This esterase 713 is encoded by a 1062 base pair gene. The presence of a leader sequence of 27 amino acids suggests that this enzyme is exported from the cytosol. Esterase 713 has been over-expressed in Agrobacterium without this leader sequence. Its amino acid sequence shows no significant homology to any known protein sequence.

PubMed: 10673440
DOI: 10.1016/S0969-2126(00)00090-3

実験手法

X-RAY DIFFRACTION (2.03 Å)