2WIM

Crystal structure of NCAM2 IG1-3

2WIM の概要

• エントリーDOI: 10.2210/pdb2wim/pdb
• 関連するPDBエントリー: 2DOC 2JLL 2V5T 2VAJ 2XY1 2XY2 2XYC
• 分子名称: NEURAL CELL ADHESION MOLECULE 2, 2-acetamido-2-deoxy-beta-D-glucopyranose, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: cell membrane, cell adhesion, transmembrane, immunoglobulin domain
• 由来する生物種: HOMO SAPIENS (HUMAN)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 65976.82

構造登録者

Kulahin, N.,Kristensen, O.,Rasmussen, K.,Kastrup, J.,Berezin, V.,Bock, E.,Walmod, P.,Gajhede, M. (登録日: 2009-05-13, 公開日: 2010-08-25, 最終更新日: 2024-10-09)

主引用文献

Kulahin, N.,Kristensen, O.,Rasmussen, K.K.,Olsen, L.,Rydberg, P.,Vestergaard, B.,Kastrup, J.S.,Berezin, V.,Bock, E.,Walmod, P.S.,Gajhede, M.
Structural model and trans-interaction of the entire ectodomain of the olfactory cell adhesion molecule.
Structure, 19:203-211, 2011

PubMed Abstract: The ectodomain of olfactory cell adhesion molecule (OCAM/NCAM2/RNCAM) consists of five immunoglobulin (Ig) domains (IgI-V), followed by two fibronectin-type 3 (Fn3) domains (Fn3I-II). A complete structural model of the entire ectodomain of human OCAM has been assembled from crystal structures of six recombinant proteins corresponding to different regions of the ectodomain. The model is the longest experimentally based composite structural model of an entire IgCAM ectodomain. It displays an essentially linear arrangement of IgI-V, followed by bends between IgV and Fn3I and between Fn3I and Fn3II. Proteins containing IgI-IgII domains formed stable homodimers in solution and in crystals. Dimerization could be disrupted in vitro by mutations in the dimer interface region. In conjunction with the bent ectodomain conformation, which can position IgI-V parallel with the cell surface, the IgI-IgII dimerization enables OCAM-mediated trans-interactions with an intercellular distance of about 20 nm, which is consistent with that observed in synapses.

PubMed: 21300289
DOI: 10.1016/j.str.2010.12.014

実験手法

X-RAY DIFFRACTION (3 Å)