2WHS

Fluorescent Protein mKeima at pH 3.8

2WHS の概要

• エントリーDOI: 10.2210/pdb2whs/pdb
• 関連するPDBエントリー: 2WHT 2WHU
• 分子名称: LARGE STOKES SHIFT FLUORESCENT PROTEIN, SULFATE ION (3 entities in total)
• 機能のキーワード: fluorescent protein, stokes shift, mkeima
• 由来する生物種: Montipora sp. 20
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 109983.44

構造登録者

Violot, S.,Carpentier, P.,Blanchoin, L.,Bourgeois, D. (登録日: 2009-05-06, 公開日: 2009-08-11, 最終更新日: 2024-11-13)

主引用文献

Violot, S.,Carpentier, P.,Blanchoin, L.,Bourgeois, D.
Reverse Ph-Dependence of Chromophore Protonation Explains the Large Stokes Shift of the Red Fluorescent Protein Mkeima.
J.Am.Chem.Soc., 131:10356-, 2009

PubMed Abstract: The recently developed red fluorescent protein Keima exhibits the largest Stokes shift (180 nm) observed to date. Combining X-ray crystallography with (in crystallo) UV-visible absorption, fluorescence, and Raman spectroscopy, we have investigated molecular determinants of this peculiar property. The results demonstrate a pH-dependent "reverse chromophore protonation" triggered by the key residue Asp157 and which couples to cis/trans isomerization of the chromophore. These data provided guidelines to rationally design a useful Keima variant.

PubMed: 19722611
DOI: 10.1021/JA903695N

実験手法

X-RAY DIFFRACTION (2.1 Å)