2WFM

Crystal structure of polyneuridine aldehyde esterase mutant (H244A)

2WFM の概要

• エントリーDOI: 10.2210/pdb2wfm/pdb
• 関連するPDBエントリー: 2WFL
• 分子名称: POLYNEURIDINE ALDEHYDE ESTERASE (2 entities in total)
• 機能のキーワード: alkaloid metabolism, monoterpenoid indole alkaloids, pnae, hydrolase, serine esterase
• 由来する生物種: RAUVOLFIA SERPENTINA (SERPENTWOOD)
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 148121.49

構造登録者

Yang, L.,Hill, M.,Panjikar, S.,Wang, M.,Stoeckigt, J. (登録日: 2009-04-08, 公開日: 2010-04-21, 最終更新日: 2023-12-13)

主引用文献

Yang, L.,Hill, M.,Wang, M.,Panjikar, S.,Stockigt, J.
Structural Basis and Enzymatic Mechanism of the Biosynthesis of C9- from C10-Monoterpenoid Indole Alkaloids.
Angew.Chem.Int.Ed.Engl., 48:5211-, 2009

PubMed Abstract: Cutting carbons: The three-dimensional structure of polyneuridine aldehyde esterase (PNAE) gives insight into the enzymatic mechanism of the biosynthesis of C(9)- from C(10)-monoterpenoid indole alkaloids (see scheme). PNAE is a very substrate-specific serine esterase. It harbors the catalytic triad S87-D216-H244, and is a new member of the alpha/beta-fold hydrolase superfamily. Its novel function leads to the diversification of alkaloid structures.

PubMed: 19496101
DOI: 10.1002/ANIE.200900150

実験手法

X-RAY DIFFRACTION (2.2 Å)