2WCR

Crystal Structure of Tip-Alpha N34 (HP0596) from Helicobacter pylori at pH8

2WCR の概要

• エントリーDOI: 10.2210/pdb2wcr/pdb
• 関連するPDBエントリー: 2WCQ
• 分子名称: TNF-ALPHA INDUCER PROTEIN, IMIDAZOLE, NICKEL (II) ION, ... (4 entities in total)
• 機能のキーワード: hp0596, tip-alpha, helicobacter pylori, immune system
• 由来する生物種: HELICOBACTER PYLORI
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36589.61

構造登録者

Tosi, T.,Cioci, G.,Jouravleva, K.,Dian, C.,Terradot, L. (登録日: 2009-03-13, 公開日: 2009-05-12, 最終更新日: 2023-12-13)

主引用文献

Tosi, T.,Cioci, G.,Jouravleva, K.,Dian, C.,Terradot, L.
Structures of the Tumor Necrosis Factor Alpha Inducing Protein Tipalpha: A Novel Virulence Factor from Helicobacter Pylori.
FEBS Lett., 583:1581-, 2009

PubMed Abstract: Helicobacter pylori secretes a unique virulence factor, Tipalpha, that enters gastric cells and both stimulates the production of the TNF-alpha and activates the NF-kappaB pathway. The structures of a truncated version of Tipalpha (TipalphaN34) in two crystal forms are presented here. Tipalpha adopts a novel beta(1)alpha(1)alpha(2)beta(2)beta(3)alpha(3)alpha(4) topology that can be described as a combination of three domains. A first region consists in a short flexible extension, a second displays a dodecin-like fold and a third is a helical bundle domain similar to the sterile alpha motif (SAM). Analysis of the oligomerisation states of TipalphaN34 in the crystals and in solution suggests that the disulfide bridges could hold together Tipalpha monomers during their secretion in the gastric environment.

PubMed: 19401200
DOI: 10.1016/J.FEBSLET.2009.04.033

実験手法

X-RAY DIFFRACTION (1.7 Å)