2WCL

Structure of BMori GOBP2 (General Odorant Binding Protein 2) with (8E, 10Z)-hexadecadien-1-ol

2WCL の概要

• エントリーDOI: 10.2210/pdb2wcl/pdb
• 関連するPDBエントリー: 2WC5 2WC6 2WCH 2WCJ 2WCK 2WCM
• 分子名称: GENERAL ODORANT-BINDING PROTEIN 1, MAGNESIUM ION, (8E,10Z)-HEXADECA-8,10-DIEN-1-OL, ... (4 entities in total)
• 機能のキーワード: odorant binding protein, olfaction, transport, transport protein, disulfide bond, insect pheremone, sensory transduction
• 由来する生物種: BOMBYX MORI (SILK MOTH)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16498.73

構造登録者

Robertson, G.,Zhou, J.-J.,He, X.,Pickett, J.A.,Field, L.M.,Keep, N.H. (登録日: 2009-03-12, 公開日: 2009-08-11, 最終更新日: 2023-12-13)

主引用文献

Zhou, J.-J.,Robertson, G.,He, X.,Dufour, S.,Hooper, A.M.,Pickett, J.A.,Keep, N.H.,Field, L.M.
Characterisation of Bombyx Mori Odorant-Binding Proteins Reveals that a General Odorant-Binding Protein Discriminates between Sex Pheromone Components.
J.Mol.Biol., 389:529-, 2009

PubMed Abstract: In many insect species, odorant-binding proteins (OBPs) are thought to be responsible for the transport of pheromones and other semiochemicals across the sensillum lymph to the olfactory receptors (ORs) within the antennal sensilla. In the silkworm Bombyx mori, the OBPs are subdivided into three main subfamilies; pheromone-binding proteins (PBPs), general odorant-binding proteins (GOBPs) and antennal-binding proteins (ABPs). We used the MotifSearch algorithm to search for genes encoding putative OBPs in B. mori and found 13, many fewer than are found in the genomes of fruit flies and mosquitoes. The 13 genes include seven new ABP-like OBPs as well as the previously identified PBPs (three), GOBPs (two) and ABPx. Quantitative examination of transcript levels showed that BmorPBP1, BmorGOBP1, BmorGOBP2 and BmorABPx are expressed at very high levels in the antennae and so could be involved in olfaction. A new two-phase binding assay, along with other established assays, showed that BmorPBP1, BmorPBP2, BmorGOBP2 and BmorABPx all bind to the B. mori sex pheromone component (10E,12Z)-hexadecadien-1-ol (bombykol). BmorPBP1, BmorPBP2 and BmorABPx also bind the pheromone component (10E,12Z)-hexadecadienal (bombykal) equally well, whereas BmorGOBP2 can discriminate between bombykol and bombykal. X-ray structures show that when bombykol is bound to BmorGOBP2 it adopts a different conformation from that found when it binds to BmorPBP1. Binding to BmorGOBP2 involves hydrogen bonding to Arg110 rather than to Ser56 as found for BmorPBP1.

PubMed: 19371749
DOI: 10.1016/J.JMB.2009.04.015

実験手法

X-RAY DIFFRACTION (1.61 Å)