2WBR

The RRM domain in GW182 proteins contributes to miRNA-mediated gene silencing

2WBR の概要

• エントリーDOI: 10.2210/pdb2wbr/pdb
• 分子名称: GW182 (1 entity in total)
• 機能のキーワード: dna-binding protein, rrm, rbd, gw182, tnrc6a, mirnas, p-bodies, argonaute, mrna decay, dna binding protein
• 由来する生物種: DROSOPHILA MELANOGASTER (FRUIT FLY)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9828.23

構造登録者

Eulalio, A.,Tritschler, F.,Buettner, R.,Weichenrieder, O.,Izaurralde, E.,Truffault, V. (登録日: 2009-03-03, 公開日: 2009-03-24, 最終更新日: 2024-05-15)

主引用文献

Eulalio, A.,Tritschler, F.,Buettner, R.,Weichenrieder, O.,Izaurralde, E.,Truffault, V.
The Rrm Domain in Gw182 Proteins Contributes to Mirna-Mediated Gene Silencing.
Nucleic Acids Res., 37:2974-, 2009

PubMed Abstract: Proteins of the GW182 family interact with Argonaute proteins and are required for miRNA-mediated gene silencing. These proteins contain two structural domains, an ubiquitin-associated (UBA) domain and an RNA recognition motif (RRM), embedded in regions predicted to be unstructured. The structure of the RRM of Drosophila melanogaster GW182 reveals that this domain adopts an RRM fold, with an additional C-terminal alpha-helix. The helix lies on the beta-sheet surface, generally used by these domains to bind RNA. This, together with the absence of aromatic residues in the conserved RNP1 and RNP2 motifs, and the lack of general affinity for RNA, suggests that the GW182 RRM does not bind RNA. The domain may rather engage in protein interactions through an unusual hydrophobic cleft exposed on the opposite face of the beta-sheet. We further show that the GW182 RRM is dispensable for P-body localization and for interaction of GW182 with Argonaute-1 and miRNAs. Nevertheless, its deletion impairs the silencing activity of GW182 in a miRNA target-specific manner, indicating that this domain contributes to silencing. The conservation of structural and surface residues suggests that the RRM domain adopts a similar fold with a related function in insect and vertebrate GW182 family members.

PubMed: 19295135
DOI: 10.1093/NAR/GKP173

実験手法

SOLUTION NMR