2W7Z

Structure of the pentapeptide repeat protein EfsQnr, a DNA gyrase inhibitor. Free amines modified by cyclic pentylation with glutaraldehyde.

2W7Z の概要

• エントリーDOI: 10.2210/pdb2w7z/pdb
• 分子名称: PENTAPEPTIDE REPEAT FAMILY PROTEIN, CHLORIDE ION (3 entities in total)
• 機能のキーワード: glutaraldehyde, gyrase inhibitor, cyclic pentylation, chemical modification, pentapeptide repeat protein, inhibitor
• 由来する生物種: ENTEROCOCCUS FAECALIS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 50188.53

構造登録者

Vetting, M.W.,Hegde, S.S.,Blanchard, J.S. (登録日: 2009-01-06, 公開日: 2009-05-05, 最終更新日: 2024-10-09)

主引用文献

Vetting, M.W.,Hegde, S.S.,Blanchard, J.S.
Crystallization of a Pentapeptide-Repeat Protein by Reductive Cyclic Pentylation of Free Amines with Glutaraldehyde.
Acta Crystallogr.,Sect.D, 65:462-, 2009

PubMed Abstract: The pentapeptide-repeat protein EfsQnr from Enterococcus faecalis protects DNA gyrase from inhibition by fluoroquinolones. EfsQnr was cloned and purified to homogeneity, but failed to produce diffraction-quality crystals in initial crystallization screens. Treatment of EfsQnr with glutaraldehyde and the strong reducing agent borane-dimethylamine resulted in a derivatized protein which produced crystals that diffracted to 1.6 A resolution; their structure was subsequently determined by single-wavelength anomalous dispersion. Analysis of the derivatized protein using Fourier transform ion cyclotron resonance mass spectrometry indicated a mass increase of 68 Da per free amino group. Electron-density maps about a limited number of structurally ordered lysines indicated that the modification was a cyclic pentylation of free amines, producing piperidine groups.

PubMed: 19390151
DOI: 10.1107/S0907444909008324

実験手法

X-RAY DIFFRACTION (1.6 Å)