2W6G

Low resolution structures of bovine mitochondrial F1-ATPase during controlled dehydration: Hydration State 3.

2W6G の概要

• エントリーDOI: 10.2210/pdb2w6g/pdb
• 関連するPDBエントリー: 1BMF 1COW 1E1Q 1E1R 1E79 1EFR 1H8E 1H8H 1NBM 1OHH 1QO1 1W0J 1W0K 2CK3 2JDI 2JIZ 2JJ1 2JJ2 2V7Q 2W6E 2W6F 2W6H 2W6I 2W6J
• 分子名称: ATP SYNTHASE SUBUNIT ALPHA HEART ISOFORM, MITOCHONDRIAL, ATP SYNTHASE SUBUNIT BETA, MITOCHONDRIAL, ATP SYNTHASE SUBUNIT GAMMA, MITOCHONDRIAL (3 entities in total)
• 機能のキーワード: atp phosphorylase (h+ transporting), transit peptide, f1fo atp phosphorylase, ion transport, mitochondrion, atp synthesis, cf(1), p-loop, hydrolase, nucleotide-binding, hydrogen ion transport, pyrrolidone carboxylic acid, atp-binding
• 由来する生物種: BOS TAURUS (BOVINE); 詳細
• 細胞内の位置: Mitochondrion inner membrane (By similarity): P19483; Mitochondrion: P00829 P05631
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 381526.11

構造登録者

Sanchez-Weatherby, J.,Felisaz, F.,Gobbo, A.,Huet, J.,Ravelli, R.B.G.,Bowler, M.W.,Cipriani, F. (登録日: 2008-12-18, 公開日: 2009-10-27, 最終更新日: 2023-12-13)

主引用文献

Sanchez-Weatherby, J.,Felisaz, F.,Gobbo, A.,Huet, J.,Ravelli, R.B.G.,Bowler, M.W.,Cipriani, F.
Improving Diffraction by Humidity Control: A Novel Device Compatible with X-Ray Beamlines.
Acta Crystallogr.,Sect.D, 65:1237-, 2009

PubMed Abstract: Dehydration of protein crystals is rarely used, despite being a post-crystallization method that is useful for the improvement of crystal diffraction properties, as it is difficult to reproduce and monitor. A novel device for hydration control of macromolecular crystals in a standard data-collection environment has been developed. The device delivers an air stream of precise relative humidity that can be used to alter the amount of water in macromolecular crystals. The device can be rapidly installed and is fully compatible with most standard synchrotron X-ray beamlines. Samples are mounted in cryoloops and the progress of dehydration can be monitored both optically and by the acquisition of diffraction images. Once the optimal hydration level has been obtained, cryocooling is easy to achieve by hand or by using a sample changer. The device has been thoroughly tested on several ESRF beamlines and is available to users.

PubMed: 19966409
DOI: 10.1107/S0907444909037822

実験手法

X-RAY DIFFRACTION (6 Å)