2W65

Anti citrullinated Collagen type 2 antibody acc4 in complex with a citrullinated peptide

2W65 の概要

• エントリーDOI: 10.2210/pdb2w65/pdb
• 関連するPDBエントリー: 2W60
• 分子名称: ANTI-CITRULLINATED COLLAGEN TYPE II FAB ACC4, COLLAGEN DERIVED PEPTIDE PCII-CIT1, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: arthritis, collagen type ii, antibody anti-citrullin, immune system
• 由来する生物種: MUS MUSCULUS (MOUSE); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 96806.26

構造登録者

Uysal, H.,Bockermann, R.,Nandakumar, K.S.,Sehnert, B.,Bajtner, E.,Engstrom, A.,Serre, G.,Burkhardt, H.,Thunnissen, M.M.G.M.,Holmdahl, R. (登録日: 2008-12-17, 公開日: 2009-02-24, 最終更新日: 2023-12-13)

主引用文献

Uysal, H.,Bockermann, R.,Nandakumar, K.S.,Sehnert, B.,Bajtner, E.,Engstrom, A.,Serre, G.,Burkhardt, H.,Thunnissen, M.M.,Holmdahl, R.
Structure and pathogenicity of antibodies specific for citrullinated collagen type II in experimental arthritis.
J. Exp. Med., 206:449-462, 2009

PubMed Abstract: Antibodies to citrulline-modified proteins have a high diagnostic value in rheumatoid arthritis (RA). However, their biological role in disease development is still unclear. To obtain insight into this question, a panel of mouse monoclonal antibodies was generated against a major triple helical collagen type II (CII) epitope (position 359-369; ARGLTGRPGDA) with or without arginines modified by citrullination. These antibodies bind cartilage and synovial tissue, and mediate arthritis in mice. Detection of citrullinated CII from RA patients' synovial fluid demonstrates that cartilage-derived CII is indeed citrullinated in vivo. The structure determination of a Fab fragment of one of these antibodies in complex with a citrullinated peptide showed a surprising beta-turn conformation of the peptide and provided information on citrulline recognition. Based on these findings, we propose that autoimmunity to CII, leading to the production of antibodies specific for both native and citrullinated CII, is an important pathogenic factor in the development of RA.

PubMed: 19204106
DOI: 10.1084/jem.20081862

実験手法

X-RAY DIFFRACTION (2.21 Å)