2W48

Crystal structure of the Full-length Sorbitol Operon Regulator SorC from Klebsiella pneumoniae

2W48 の概要

• エントリーDOI: 10.2210/pdb2w48/pdb
• 分子名称: SORBITOL OPERON REGULATOR, GLYCEROL, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (6 entities in total)
• 機能のキーワード: sorc, activator, repressor, dna-binding, transcription, klebsiella pneumoniae, transcription regulator, transcription regulation
• 由来する生物種: KLEBSIELLA PNEUMONIAE
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 141031.42

構造登録者

de Sanctis, D.,McVey, C.E.,Enguita, F.J.,Carrondo, M.A. (登録日: 2008-11-21, 公開日: 2009-05-05, 最終更新日: 2023-12-13)

主引用文献

De Sanctis, D.,Mcvey, C.E.,Enguita, F.J.,Carrondo, M.A.
Crystal Structure of the Full-Length Sorbitol Operon Regulator Sorc from Klebsiella Pneumoniae: Structural Evidence for a Novel Transcriptional Regulation Mechanism.
J.Mol.Biol., 387:759-, 2009

PubMed Abstract: SorC transcriptional regulators are common regulators in prokaryotes. Here we report the first crystal structure of a full-length SorC, the sorbitol operon regulator SorC from Klebsiella pneumoniae, the prototype of its family. SorC was found to be a homotetramer (which seems to be the biologically active form) that is able to recognize its DNA operator. The tetramer can be regarded as a dimer of dimers, with each dimer being composed of two identical subunits in different conformations. The DNA-binding domains divergently protrude from the core of the tetramer, suggesting that SorC may bind its operator in two distinct regions. The sugar-binding domain presents the same fold identified in members of the SorC family that shows some features identified as specific for sugar recognition. An in silico analysis shows that the localization of the putative sugar-binding site is close to the dimeric interfaces. This supports the proposal of a new mechanism of transcriptional regulation that is in complete agreement with functional studies recently reported on a protein belonging to the same family.

PubMed: 19232357
DOI: 10.1016/J.JMB.2009.02.017

実験手法

X-RAY DIFFRACTION (3.2 Å)