2W42

THE STRUCTURE OF A PIWI PROTEIN FROM ARCHAEOGLOBUS FULGIDUS COMPLEXED WITH A 16NT DNA DUPLEX.

2W42 の概要

• エントリーDOI: 10.2210/pdb2w42/pdb
• 関連するPDBエントリー: 1W9H 2BGG
• 分子名称: PUTATIVE UNCHARACTERIZED PROTEIN, 5'-D(*TP*TP*CP*GP*AP*CP*GP*CP)-3', 5'-D(*GP*TP*CP*GP*AP*AP*TP*TP)-3', ... (5 entities in total)
• 機能のキーワード: rnai, risc, argonaute, piwi domain, protein-dna complex, protein-dna complex complex, protein/dna complex
• 由来する生物種: ARCHAEOGLOBUS FULGIDUS; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 108403.18

構造登録者

Parker, J.S.,Roe, S.M.,Barford, D. (登録日: 2008-11-19, 公開日: 2008-12-09, 最終更新日: 2023-12-13)

主引用文献

Parker, J.S.,Parizotto, E.A.,Wang, M.,Roe, S.M.,Barford, D.
Enhancement of the Seed-Target Recognition Step in RNA Silencing by a Piwi-Mid Domain Protein
Mol.Cell, 33:204-, 2009

PubMed Abstract: Target recognition in RNA silencing is governed by the "seed sequence" of a guide RNA strand associated with the PIWI/MID domain of an Argonaute protein in RISC. Using a reconstituted in vitro target recognition system, we show that a model PIWI/MID domain protein confers position-dependent tightening and loosening of guide-strand-target interactions. Over the seed sequence, the interaction affinity is enhanced up to approximately 300-fold. Enhancement is achieved through a reduced entropy penalty for the interaction. In contrast, interactions 3' of the seed are inhibited. We quantified mismatched target recognition inside and outside the seed, revealing amplified discrimination at the third position in the seed mediated by the PIWI/MID domain. Thus, association of the guide strand with the PIWI/MID domain generates an enhanced affinity anchor site over the seed that can promote fidelity in target recognition and stabilize and guide the assembly of the active silencing complex.

PubMed: 19187762
DOI: 10.1016/J.MOLCEL.2008.12.012

実験手法

X-RAY DIFFRACTION (1.9 Å)