2W31

globin domain of Geobacter sulfurreducens globin-coupled sensor

2W31 の概要

• エントリーDOI: 10.2210/pdb2w31/pdb
• 分子名称: GLOBIN, PROTOPORPHYRIN IX CONTAINING FE, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: oxygen transport, geobacter sulfurreducens, hexacoordination, globin coupled sensor
• 由来する生物種: GEOBACTER SULFURREDUCENS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 39890.72

構造登録者

Pesce, A.,Thijs, L.,Nardini, M.,Desmet, F.,Sisinni, L.,Gourlay, L.,Bolli, A.,Coletta, M.,Van Doorslaer, S.,Wan, X.,Alam, M.,Ascenzi, P.,Moens, L.,Bolognesi, M.,Dewilde, S. (登録日: 2008-11-05, 公開日: 2009-01-13, 最終更新日: 2025-10-01)

主引用文献

Pesce, A.,Thijs, L.,Nardini, M.,Desmet, F.,Sisinni, L.,Gourlay, L.,Bolli, A.,Coletta, M.,Van Doorslaer, S.,Wan, X.,Alam, M.,Ascenzi, P.,Moens, L.,Bolognesi, M.,Dewilde, S.
Hise11 and Hisf8 Provide Bis-Histidyl Heme Hexa-Coordination in the Globin Domain of Geobacter Sulfurreducens Globin-Coupled Sensor.
J.Mol.Biol., 386:246-, 2009

PubMed Abstract: Among heme-based sensors, recent phylogenomic and sequence analyses have identified 34 globin coupled sensors (GCS), to which an aerotactic or gene-regulating function has been tentatively ascribed. Here, the structural and biochemical characterization of the globin domain of the GCS from Geobacter sulfurreducens (GsGCS(162)) is reported. A combination of X-ray crystallography (crystal structure at 1.5 A resolution), UV-vis and resonance Raman spectroscopy reveals the ferric GsGCS(162) as an example of bis-histidyl hexa-coordinated GCS. In contrast to the known hexa-coordinated globins, the distal heme-coordination in ferric GsGCS(162) is provided by a His residue unexpectedly located at the E11 topological site. Furthermore, UV-vis and resonance Raman spectroscopy indicated that ferrous deoxygenated GsGCS(162) is a penta-/hexa-coordinated mixture, and the heme hexa-to-penta-coordination transition does not represent a rate-limiting step for carbonylation kinetics. Lastly, electron paramagnetic resonance indicates that ferrous nitrosylated GsGCS(162) is a penta-coordinated species, where the proximal HisF8-Fe bond is severed.

PubMed: 19109973
DOI: 10.1016/J.JMB.2008.12.023

実験手法

X-RAY DIFFRACTION (1.5 Å)