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2W10

Mona SH3C in complex

Summary for 2W10
Entry DOI10.2210/pdb2w10/pdb
Related1H3H 1OEB 1R1P 1R1Q 1R1S 1UTI 2D0N
DescriptorGRB2-RELATED ADAPTOR PROTEIN 2, TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 23, PHOSPHATE ION, ... (4 entities in total)
Functional Keywordsalternative splicing, tpr repeat, sh2 domain, sh3 domain, coiled coil, protein phosphatase, cytoplasmic vesicle, phosphoprotein, signal tranduction, sh3 domain-complex, sh3, gads, mona, dimer, hd-ptp, hydrolase, cytoplasm
Biological sourceMUS MUSCULUS (MOUSE)
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Cellular locationCytoplasmic vesicle (By similarity): Q6PB44
Total number of polymer chains4
Total formula weight16939.03
Authors
Harkiolaki, M.,Feller, S.M. (deposition date: 2008-10-13, release date: 2009-05-19, Last modification date: 2023-12-13)
Primary citationHarkiolaki, M.,Tsirka, T.,Lewitzky, M.,Simister, P.C.,Joshi, D.,Bird, L.E.,Jones, E.Y.,O'Reilly, N.,Feller, S.M.
Distinct Binding Modes of Two Epitopes in Gab2 that Interact with the Sh3C Domain of Grb2.
Structure, 17:809-, 2009
Cited by
PubMed Abstract: Grb2 and Gab2 form a complex implicated in normal cell signaling and cancer development. Binding of the Grb2SH3C domain to Gab2 is essential for the interaction, but molecular details remained undefined. Using peptide arrays and isothermal titration calorimetry, two Grb2SH3C binding sites in Gab2 (Gab2a and Gab2b) were confirmed and characterized. Gab2a bears similarity to a p27Kip1 epitope that also binds Grb2SH3C. Crystal structures of both Gab2 epitopes complexed with Grb2SH3C reveal that Gab2b contains a 3(10) helix that positions the arginine and lysine of the core-binding motif RxxK in parallel orientation. In contrast, the Gab2a RxxK motif is embedded in a PPII helix with Arg and Lys in staggered orientation. A similar interaction mode is also present in a new complex of Mona/GadsSH3C with an RxxxxK epitope from the putative phosphatase HD-PTP. In summary, our study reveals interaction types of SH3 domains, highlighting their great versatility.
PubMed: 19523899
DOI: 10.1016/J.STR.2009.03.017
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.9 Å)
Structure validation

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