2W07

Structural determinants of polymerization reactivity of the P pilus adaptor subunit PapF

2W07 の概要

• エントリーDOI: 10.2210/pdb2w07/pdb
• 分子名称: CHAPERONE PROTEIN PAPD, MINOR PILIN SUBUNIT PAPF, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: donor strand complementation, nte, papd, papf, pili, pilin, groove, subunit, immunoglobulin domain, donor-strand exchange, secreted, fimbrium, periplasm, p5 pocket, chaperone, cell adhesion, cell projection, pilus biogenesis, order of assembly, n-terminal extension
• 由来する生物種: ESCHERICHIA COLI; 詳細
• 細胞内の位置: Periplasm (By similarity): Q1R2W9
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 40548.61

構造登録者

Verger, D.,Rose, R.J.,Paci, E.,Costakes, G.,Daviter, T.,Hultgren, S.,Remaut, H.,Ashcroft, A.E.,Radford, S.E.,Waksman, G. (登録日: 2008-08-12, 公開日: 2008-11-25, 最終更新日: 2024-11-06)

主引用文献

Verger, D.,Rose, R.J.,Paci, E.,Costakes, G.,Daviter, T.,Hultgren, S.,Remaut, H.,Ashcroft, A.E.,Radford, S.E.,Waksman, G.
Structural Determinants of Polymerization Reactivity of the P Pilus Adaptor Subunit Papf.
Structure, 16:1724-, 2008

PubMed Abstract: P pili are important adhesive fibers involved in kidney infection by uropathogenic Escherichia coli. Pilus subunits are characterized by a large groove resulting from lack of a beta strand. Polymerization of pilus subunits occurs via the donor-strand exchange (DSE) mechanism initiated when the N terminus of an incoming subunit interacts with the P5 region/pocket of the previously assembled subunit groove. Here, we solve the structure of the PapD:PapF complex in order to understand why PapF undergoes slow DSE. The structure reveals that the PapF P5 pocket is partially obstructed. MD simulations show this region of PapF is flexible compared with its equivalent in PapH, a subunit that also has an obstructed P5 pocket and is unable to undergo DSE. Using electrospray-ionization mass spectrometry, we show that mutations in the P5 region result in increased DSE rates. Thus, partial obstruction of the P5 pocket serves as a modulating mechanism of DSE.

PubMed: 19000824
DOI: 10.1016/J.STR.2008.08.012

実験手法

X-RAY DIFFRACTION (2.2 Å)