2VY9

Molecular architecture of the stressosome, a signal integration and transduction hub

Summary for 2VY9

• Entry DOI: 10.2210/pdb2vy9/pdb
• Descriptor: ANTI-SIGMA-FACTOR ANTAGONIST (2 entities in total)
• Functional Keywords: moorella thermoacetica, gene regulation, rsbs, stressosome, stas domain, bacillus subtilis
• Biological source: MOORELLA THERMOACETICA
• Total number of polymer chains: 2
• Total formula weight: 27537.09

Authors

Marles-Wright, J.,Grant, T.,Delumeau, O.,van Duinen, G.,Firbank, S.J.,Lewis, P.J.,Murray, J.W.,Newman, J.A.,Quin, M.B.,Race, P.R.,Rohou, A.,Tichelaar, W.,van Heel, M.,Lewis, R.J. (deposition date: 2008-07-21, release date: 2008-10-14, Last modification date: 2024-10-16)

Primary citation

Marles-Wright, J.,Grant, T.,Delumeau, O.,Van Duinen, G.,Firbank, S.J.,Lewis, P.J.,Murray, J.W.,Newman, J.A.,Quin, M.B.,Race, P.R.,Rohou, A.,Tichelaar, W.,Van Heel, M.,Lewis, R.J.
Molecular Architecture of the "Stressosome," a Signal Integration and Transduction Hub
Science, 322:92-, 2008

PubMed Abstract: A commonly used strategy by microorganisms to survive multiple stresses involves a signal transduction cascade that increases the expression of stress-responsive genes. Stress signals can be integrated by a multiprotein signaling hub that responds to various signals to effect a single outcome. We obtained a medium-resolution cryo-electron microscopy reconstruction of the 1.8-megadalton "stressosome" from Bacillus subtilis. Fitting known crystal structures of components into this reconstruction gave a pseudoatomic structure, which had a virus capsid-like core with sensory extensions. We suggest that the different sensory extensions respond to different signals, whereas the conserved domains in the core integrate the varied signals. The architecture of the stressosome provides the potential for cooperativity, suggesting that the response could be tuned dependent on the magnitude of chemophysical insult.

PubMed: 18832644
DOI: 10.1126/SCIENCE.1159572

Experimental method

X-RAY DIFFRACTION (2.3 Å)