2VY2

Structure of LEAFY transcription factor from Arabidopsis thaliana in complex with DNA from AG-I promoter

2VY2 の概要

• エントリーDOI: 10.2210/pdb2vy2/pdb
• 関連するPDBエントリー: 2VY1
• 分子名称: PROTEIN LEAFY, 5'-D(*AP*TP*TP*TP*AP*AP*TP*CP*CP*AP *AP*TP*GP*GP*TP*TP*AP*CP*AP*A)-3' (3 entities in total)
• 機能のキーワード: dna-binding, polymorphism, transcription, developmental protein transcription regulation, homeotic genes, activator, differentiation flower development, flowering, coiled coil genes
• 由来する生物種: ARABIDOPSIS THALIANA (MOUSE-EAR CRESS); 詳細
• 細胞内の位置: Nucleus (Probable): Q00958
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 28008.01

構造登録者

Hames, C.,Ptchelkine, D.,Grimm, C.,Thevenon, E.,Moyroud, E.,Gerard, F.,Martiel, J.L.,Benlloch, R.,Parcy, F.,Muller, C.W. (登録日: 2008-07-16, 公開日: 2008-09-23, 最終更新日: 2023-12-13)

主引用文献

Hames, C.,Ptchelkine, D.,Grimm, C.,Thevenon, E.,Moyroud, E.,Gerard, F.,Martiel, J.L.,Benlloch, R.,Parcy, F.,Muller, C.W.
Structural Basis for Leafy Floral Switch Function and Similarity with Helix-Turn-Helix Proteins.
Embo J., 27:2628-, 2008

PubMed Abstract: The LEAFY (LFY) protein is a key regulator of flower development in angiosperms. Its gradually increased expression governs the sharp floral transition, and LFY subsequently controls the patterning of flower meristems by inducing the expression of floral homeotic genes. Despite a wealth of genetic data, how LFY functions at the molecular level is poorly understood. Here, we report crystal structures for the DNA-binding domain of Arabidopsis thaliana LFY bound to two target promoter elements. LFY adopts a novel seven-helix fold that binds DNA as a cooperative dimer, forming base-specific contacts in both the major and minor grooves. Cooperativity is mediated by two basic residues and plausibly accounts for LFY's effectiveness in triggering sharp developmental transitions. Our structure reveals an unexpected similarity between LFY and helix-turn-helix proteins, including homeodomain proteins known to regulate morphogenesis in higher eukaryotes. The appearance of flowering plants has been linked to the molecular evolution of LFY. Our study provides a unique framework to elucidate the molecular mechanisms underlying floral development and the evolutionary history of flowering plants.

PubMed: 18784751
DOI: 10.1038/EMBOJ.2008.184

実験手法

X-RAY DIFFRACTION (2.3 Å)