2VXI

The binding of heme and zinc in Escherichia coli Bacterioferritin

Summary for 2VXI

Entry DOI	10.2210/pdb2vxi/pdb
Related	1BCF 1BFR 2HTN
Descriptor	BACTERIOFERRITIN, PROTOPORPHYRIN IX CONTAINING FE, ZINC ION, ... (5 entities in total)
Functional Keywords	metal transport, bacterioferritin, iron storage and electron transport, zinc, heme, iron, iron storage, metal-binding
Biological source	ESCHERICHIA COLI
Total number of polymer chains	12
Total formula weight	233969.68
Authors	Willies, S.C.,Isupov, M.N.,Garman, E.F.,Littlechild, J.A. (deposition date: 2008-07-04, release date: 2008-11-04, Last modification date: 2024-05-08)
Primary citation	Willies, S.C.,Isupov, M.N.,Garman, E.F.,Littlechild, J.A. The Binding of Haem and Zinc in the 1.9 A X-Ray Structure of Escherichia Coli Bacterioferritin. J.Biol.Inorg.Chem., 14:201-, 2009 Cited by PubMed Abstract: The crystal structure of Escherichia coli bacterioferritin has been solved to 1.9 A, and shows the symmetrical binding of a haem molecule on the local twofold axis between subunits and a pair of metal atoms bound to each subunit at the ferroxidase centre. These metals have been identified as zinc by the analysis of the structure and X-ray data and confirmed by microfocused proton-induced X-ray emission experiments. For the first time the haem has been shown to be linked to both the internal and the external environments via a cluster of waters positioned above the haem molecule. PubMed: 18946693 DOI: 10.1007/S00775-008-0438-8 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.91 Å)

Structure validation