2VXI

The binding of heme and zinc in Escherichia coli Bacterioferritin

2VXI の概要

• エントリーDOI: 10.2210/pdb2vxi/pdb
• 関連するPDBエントリー: 1BCF 1BFR 2HTN
• 分子名称: BACTERIOFERRITIN, PROTOPORPHYRIN IX CONTAINING FE, ZINC ION, ... (5 entities in total)
• 機能のキーワード: metal transport, bacterioferritin, iron storage and electron transport, zinc, heme, iron, iron storage, metal-binding
• 由来する生物種: ESCHERICHIA COLI
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 233969.68

構造登録者

Willies, S.C.,Isupov, M.N.,Garman, E.F.,Littlechild, J.A. (登録日: 2008-07-04, 公開日: 2008-11-04, 最終更新日: 2024-05-08)

主引用文献

Willies, S.C.,Isupov, M.N.,Garman, E.F.,Littlechild, J.A.
The Binding of Haem and Zinc in the 1.9 A X-Ray Structure of Escherichia Coli Bacterioferritin.
J.Biol.Inorg.Chem., 14:201-, 2009

PubMed Abstract: The crystal structure of Escherichia coli bacterioferritin has been solved to 1.9 A, and shows the symmetrical binding of a haem molecule on the local twofold axis between subunits and a pair of metal atoms bound to each subunit at the ferroxidase centre. These metals have been identified as zinc by the analysis of the structure and X-ray data and confirmed by microfocused proton-induced X-ray emission experiments. For the first time the haem has been shown to be linked to both the internal and the external environments via a cluster of waters positioned above the haem molecule.

PubMed: 18946693
DOI: 10.1007/S00775-008-0438-8

実験手法

X-RAY DIFFRACTION (1.91 Å)