2VVJ

IrisFP fluorescent protein in its red form, cis conformation

これはPDB形式変換不可エントリーです。

2VVJ の概要

• エントリーDOI: 10.2210/pdb2vvj/pdb
• 関連するPDBエントリー: 1ZUX 2BTJ 2VVH 2VVI
• 分子名称: Green to red photoconvertible GFP-like protein EosFP, SULFATE ION, SULFITE ION, ... (5 entities in total)
• 機能のキーワード: photoactivation, photoconversion, fluorescent protein, optical highlighters, microspectrophotometry, eosfp, photochromism, photoswitching
• 由来する生物種: Lobophyllia hemprichii (Lobed brain coral); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 105722.92

構造登録者

Adam, V.,Lelimousin, M.,Boehme, S.,Desfonds, G.,Nienhaus, K.,Field, M.J.,Wiedenmann, J.,McSweeney, S.,Nienhaus, G.U.,Bourgeois, D. (登録日: 2008-06-09, 公開日: 2008-08-12, 最終更新日: 2023-12-13)

主引用文献

Adam, V.,Lelimousin, M.,Boehme, S.,Desfonds, G.,Nienhaus, K.,Field, M.J.,Wiedenmann, J.,Mcsweeney, S.,Nienhaus, G.U.,Bourgeois, D.
Structural Characterization of Irisfp, an Optical Highlighter Undergoing Multiple Photo-Induced Transformations.
Proc.Natl.Acad.Sci.USA, 105:18343-, 2008

PubMed Abstract: Photoactivatable fluorescent proteins (FPs) are powerful fluorescent highlighters in live cell imaging and offer perspectives for optical nanoscopy and the development of biophotonic devices. Two types of photoactivation are currently being distinguished, reversible photoswitching between fluorescent and nonfluorescent forms and irreversible photoconversion. Here, we have combined crystallography and (in crystallo) spectroscopy to characterize the Phe-173-Ser mutant of the tetrameric variant of EosFP, named IrisFP, which incorporates both types of phototransformations. In its green fluorescent state, IrisFP displays reversible photoswitching, which involves cis-trans isomerization of the chromophore. Like its parent protein EosFP, IrisFP also photoconverts irreversibly to a red-emitting state under violet light because of an extension of the conjugated pi-electron system of the chromophore, accompanied by a cleavage of the polypeptide backbone. The red form of IrisFP exhibits a second reversible photoswitching process, which may also involve cis-trans isomerization of the chromophore. Therefore, IrisFP displays altogether 3 distinct photoactivation processes. The possibility to engineer and precisely control multiple phototransformations in photoactivatable FPs offers exciting perspectives for the extension of the fluorescent protein toolkit.

PubMed: 19017808
DOI: 10.1073/PNAS.0805949105

実験手法

X-RAY DIFFRACTION (2 Å)