2VS8

The crystal structure of I-DmoI in complex with DNA and Mn

Summary for 2VS8

Related1MOW 1B24 2VS7 2VS9
DescriptorHOMING ENDONUCLEASE I-DMOI, 5'-D(*GP*CP*CP*TP*TP*GP*CP*CP*GP*GP *GP*TP*AP*A)-3', 5'-D(*GP*TP*TP*CP*CP*GP*GP*CP* DGP*DCP*DGP)-3', ... (8 entities in total)
Functional Keywordsmeganuclease, intron homing, genetic engineering, homing endonuclease, protein/dna crystallography, nuclease, hydrolase, magnesium, endonuclease, dna-binding protein, dna binding protein
Biological sourceDESULFUROCOCCUS MOBILIS
Total number of polymer chains15
Total molecular weight116514.35
Authors
Marcaida, M.J.,Prieto, J.,Redondo, P.,Nadra, A.D.,Alibes, A.,Serrano, L.,Grizot, S.,Duchateau, P.,Paques, F.,Blanco, F.J.,Montoya, G. (deposition date: 2008-04-21, release date: 2008-11-11, Last modification date: 2011-07-13)
Primary citation
Marcaida, M.J.,Prieto, J.,Redondo, P.,Nadra, A.D.,Alibes, A.,Serrano, L.,Grizot, S.,Duchateau, P.,Paques, F.,Blanco, F.J.,Montoya, G.
Crystal Structure of I-Dmoi in Complex with its Target DNA Provides New Insights Into Meganuclease Engineering.
Proc.Natl.Acad.Sci.USA, 105:16888-, 2008
PubMed: 18974222 (PDB entries with the same primary citation)
DOI: 10.1073/PNAS.0804795105
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.1 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.23590.4%2.8%6.1%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution