2VM4

Structure of Alcaligenes xylosoxidans nitrite reductase in space group R3 - 2 of 2

2VM4 の概要

• エントリーDOI: 10.2210/pdb2vm4/pdb
• 関連するPDBエントリー: 1BQ5 1GS6 1GS7 1GS8 1HAU 1HAW 1NDT 1OE1 1OE2 1OE3 1WA0 1WA1 1WA2 1WAE 2BO0 2BP0 2BP8 2JFC 2VM3
• 分子名称: DISSIMILATORY COPPER-CONTAINING NITRITE REDUCTASE, COPPER (II) ION, ZINC ION, ... (5 entities in total)
• 機能のキーワード: denitrification, nitrite reductase, oxidoreductase, ordered mechanism
• 由来する生物種: ACHROMOBACTER XYLOSOXIDANS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36957.25

構造登録者

Hough, M.A.,Antonyuk, S.V.,Strange, R.W.,Eady, R.R.,Hasnain, S.S. (登録日: 2008-01-22, 公開日: 2008-02-05, 最終更新日: 2023-12-13)

主引用文献

Hough, M.A.,Antonyuk, S.V.,Strange, R.W.,Eady, R.R.,Hasnain, S.S.
Crystallography with Online Optical and X-Ray Absorption Spectroscopies Demonstrates an Ordered Mechanism in Copper Nitrite Reductase.
J.Mol.Biol., 378:353-, 2008

PubMed Abstract: Nitrite reductases are key enzymes that perform the first committed step in the denitrification process and reduce nitrite to nitric oxide. In copper nitrite reductases, an electron is delivered from the type 1 copper (T1Cu) centre to the type 2 copper (T2Cu) centre where catalysis occurs. Despite significant structural and mechanistic studies, it remains controversial whether the substrates, nitrite, electron and proton are utilised in an ordered or random manner. We have used crystallography, together with online X-ray absorption spectroscopy and optical spectroscopy, to show that X-rays rapidly and selectively photoreduce the T1Cu centre, but that the T2Cu centre does not photoreduce directly over a typical crystallographic data collection time. Furthermore, internal electron transfer between the T1Cu and T2Cu centres does not occur, and the T2Cu centre remains oxidised. These data unambiguously demonstrate an 'ordered' mechanism in which electron transfer is gated by binding of nitrite to the T2Cu. Furthermore, the use of online multiple spectroscopic techniques shows their value in assessing radiation-induced redox changes at different metal sites and demonstrates the importance of ensuring the correct status of redox centres in a crystal structure determination. Here, optical spectroscopy has shown a very high sensitivity for detecting the change in T1Cu redox state, while X-ray absorption spectroscopy has reported on the redox status of the T2Cu site, as this centre has no detectable optical absorption.

PubMed: 18353369
DOI: 10.1016/J.JMB.2008.01.097

実験手法

X-RAY DIFFRACTION (1.9 Å)