2VL5

Structure of anti-collagen type II FAb CIIC1

Summary for 2VL5

• Entry DOI: 10.2210/pdb2vl5/pdb
• Descriptor: CIIC1 ANTICOLLAGEN FAB (3 entities in total)
• Functional Keywords: rheumatoid factor, immune system, arthritis, autoantibodies, collagen type ii
• Biological source: MUS MUSCULUS (MOUSE); More
• Total number of polymer chains: 4
• Total formula weight: 94943.65

Authors

Uysal, H.,Sehnert, B.,Nandakumar, K.S.,Boiers, U.,Burkhardt, H.,Holmdahl, R.,Thunnissen, M.M. (deposition date: 2008-01-08, release date: 2008-03-18, Last modification date: 2024-10-16)

Primary citation

Uysal, H.,Sehnert, B.,Nandakumar, K.S.,Boiers, U.,Burkhardt, H.,Holmdahl, R.,Thunnissen, M.M.
The Crystal Structure of the Pathogenic Collagen Type II-Specific Mouse Monoclonal Antibody Ciic1 Fab: Structure to Function Analysis.
Mol.Immunol., 45:2196-, 2008

PubMed Abstract: Monoclonal anti-collagen type II antibody CIIC1 is an arthritogenic autoantibody, which induces arthritis in mice. We crystallized and solved the structure of CIIC1 Fab molecule. Analysis of structure revealed an interaction between the CDR regions of one Fab to the CH1 domain of another Fab, which resembles an antibody-antigen interaction. ELISA experiments confirmed the cross-reactivity of both the full CIIC1 antibody and a single chain Fv fragment to other anti-collagen antibodies which are of different isotypes and epitope specificity. The rheumatoid factor like reactivity of CIIC1 antibody together with its collagen type II specificity may explain the pathogenicity of this antibody.

PubMed: 18241923
DOI: 10.1016/J.MOLIMM.2007.12.005

Experimental method

X-RAY DIFFRACTION (2.1 Å)