2VL5

Structure of anti-collagen type II FAb CIIC1

2VL5 の概要

• エントリーDOI: 10.2210/pdb2vl5/pdb
• 分子名称: CIIC1 ANTICOLLAGEN FAB (3 entities in total)
• 機能のキーワード: rheumatoid factor, immune system, arthritis, autoantibodies, collagen type ii
• 由来する生物種: MUS MUSCULUS (MOUSE); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 94943.65

構造登録者

Uysal, H.,Sehnert, B.,Nandakumar, K.S.,Boiers, U.,Burkhardt, H.,Holmdahl, R.,Thunnissen, M.M. (登録日: 2008-01-08, 公開日: 2008-03-18, 最終更新日: 2024-10-16)

主引用文献

Uysal, H.,Sehnert, B.,Nandakumar, K.S.,Boiers, U.,Burkhardt, H.,Holmdahl, R.,Thunnissen, M.M.
The Crystal Structure of the Pathogenic Collagen Type II-Specific Mouse Monoclonal Antibody Ciic1 Fab: Structure to Function Analysis.
Mol.Immunol., 45:2196-, 2008

PubMed Abstract: Monoclonal anti-collagen type II antibody CIIC1 is an arthritogenic autoantibody, which induces arthritis in mice. We crystallized and solved the structure of CIIC1 Fab molecule. Analysis of structure revealed an interaction between the CDR regions of one Fab to the CH1 domain of another Fab, which resembles an antibody-antigen interaction. ELISA experiments confirmed the cross-reactivity of both the full CIIC1 antibody and a single chain Fv fragment to other anti-collagen antibodies which are of different isotypes and epitope specificity. The rheumatoid factor like reactivity of CIIC1 antibody together with its collagen type II specificity may explain the pathogenicity of this antibody.

PubMed: 18241923
DOI: 10.1016/J.MOLIMM.2007.12.005

実験手法

X-RAY DIFFRACTION (2.1 Å)