2VKG

COMPLEXES OF DODECIN WITH FLAVIN AND FLAVIN-LIKE LIGANDS

Summary for 2VKG

• Entry DOI: 10.2210/pdb2vkg/pdb
• Related: 1MOG 2CC6 2CC7 2CC8 2CC9 2CCB 2CCC 2CIE 2CIF 2CJC 2VKF
• Descriptor: DODECIN, MAGNESIUM ION, SODIUM ION, ... (7 entities in total)
• Functional Keywords: biotechnological application of dodecin binding properties, flavin-dna ligand hybrid, electron transport
• Biological source: HALOBACTERIUM SALINARUM
• Total number of polymer chains: 1
• Total formula weight: 7282.82

Authors

Grininger, M.,Noell, G.,Trawoeger, S.,Sinner, E.,Oesterhelt, D. (deposition date: 2007-12-19, release date: 2008-09-30, Last modification date: 2023-12-13)

Primary citation

Grininger, M.,Noell, G.,Trawoeger, S.,Sinner, E.,Oesterhelt, D.
Electrochemical Switching of the Flavoprotein Dodecin at Gold Surfaces Modified by Flavin-DNA Hybrid Linkers
Biointerphases, 3:51-, 2008

PubMed Abstract: Dodecin from Halobacterium salinarum is a dodecameric, hollow-spherical protein, which unspecifically adopts flavin molecules. Reduction of flavin dodecin holocomplexes induces dissociation into apododecin and free flavin. Unspecific binding and dissociation upon reduction were used as key properties to construct an electrochemically switchable surface, which was able to bind and release dodecin apoprotein depending on the applied potential. A flavin modified electrode surface (electrode-DNA-flavin) was generated by direct adsorption of double stranded DNA (ds-DNA) equipped with flavin and disulfide modifications at opposite ends. While the disulfide functionality enabled anchoring the ds-DNA at the gold surface, the flavin exposed at the surface served as the redox-active dodecin docking site. The structures of protein and flavin-DNA hybrid ligands were optimized and characterized by x-ray structural analysis of the holocomplexes. By surface plasmon resonance (SPR) spectroscopy, the adsorption of flavin modified DNA as well as the binding and the electrochemically induced release of dodecin apoprotein could be shown. When the surface immobilization protocol was changed from direct immobilization of the modified ds-DNA to a protocol, which included the hybridization of flavin and thiol modified DNA at the surface, the resulting monolayer was electrochemically inactive. A possible explanation for the strong influence of the surface immobilization protocol on addressing dodecin by the applied potential is that electron transfer is rather mediated by defects in the monolayer than modified ds-DNA.

PubMed: 20408700
DOI: 10.1116/1.2965134

Experimental method

X-RAY DIFFRACTION (1.8 Å)