2VJM

Formyl-CoA transferase with aspartyl-formyl anhydide intermediate

2VJM の概要

• エントリーDOI: 10.2210/pdb2vjm/pdb
• 関連するPDBエントリー: 1P5H 1P5R 1T3Z 1T4C 1VGQ 1VGR 2VJK 2VJL 2VJN 2VJO 2VJP 2VJQ
• 分子名称: FORMYL-COENZYME A TRANSFERASE, COENZYME A, SODIUM ION, ... (6 entities in total)
• 機能のキーワード: cytoplasm, transferase, class iii coa transferase
• 由来する生物種: OXALOBACTER FORMIGENES; 詳細
• 細胞内の位置: Cytoplasm : O06644 O06644
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 96550.09

構造登録者

Berthold, C.L.,Toyota, C.G.,Richards, N.G.J.,Lindqvist, Y. (登録日: 2007-12-11, 公開日: 2007-12-25, 最終更新日: 2023-12-13)

主引用文献

Berthold, C.L.,Toyota, C.G.,Richards, N.G.J.,Lindqvist, Y.
Reinvestigation of the Catalytic Mechanism of Formyl-Coa Transferase, a Class III Coa-Transferase.
J.Biol.Chem., 283:6519-, 2008

PubMed Abstract: Formyl-coenzyme A transferase from Oxalobacter formigenes belongs to the Class III coenzyme A transferase family and catalyzes the reversible transfer of a CoA carrier between formyl-CoA and oxalate, forming oxalyl-CoA and formate. Formyl-CoA transferase has a unique three-dimensional fold composed of two interlaced subunits locked together like rings of a chain. We here present an intermediate in the reaction, formyl-CoA transferase containing the covalent beta-aspartyl-CoA thioester, adopting different conformations in the two active sites of the dimer, which was identified through crystallographic freeze-trapping experiments with formyl-CoA and oxalyl-CoA in the absence of acceptor carboxylic acid. The formation of the enzyme-CoA thioester was also confirmed by mass spectrometric data. Further structural data include a trapped aspartyl-formyl anhydride protected by a glycine loop closing down over the active site. In a crystal structure of the beta-aspartyl-CoA thioester of an inactive mutant variant, oxalate was found bound to the open conformation of the glycine loop. Together with hydroxylamine trapping experiments and kinetic as well as mutagenesis data, the structures of these formyl-CoA transferase complexes provide new information on the Class III CoA-transferase family and prompt redefinition of the catalytic steps and the modified reaction mechanism of formyl-CoA transferase proposed here.

PubMed: 18162462
DOI: 10.1074/JBC.M709353200

実験手法

X-RAY DIFFRACTION (1.89 Å)