2VEQ

Insights into kinetochore-DNA interactions from the structure of Cep3p

2VEQ の概要

• エントリーDOI: 10.2210/pdb2veq/pdb
• 分子名称: CENTROMERE DNA-BINDING PROTEIN COMPLEX CBF3 SUBUNIT B, BETA-MERCAPTOETHANOL, CACODYLATE ION, ... (4 entities in total)
• 機能のキーワード: transcription factor, cell cycle, zinc, cep3p, nucleus, centromere, dna-binding, phosphorylation, chromosomal protein, kinetochore, cbf3 complex, metal-binding
• 由来する生物種: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST)
• 細胞内の位置: Nucleus : P40969
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 66593.94

構造登録者

Purvis, A.,Singleton, M.R. (登録日: 2007-10-26, 公開日: 2007-12-25, 最終更新日: 2025-04-09)

主引用文献

Purvis, A.,Singleton, M.R.
Insights Into Kinetochore-DNA Interactions from the Structure of Cep3Delta
Embo Rep., 9:56-, 2008

PubMed Abstract: The CBF3 complex is an essential core component of the budding yeast kinetochore and is required for the centromeric localization of all other kinetochore proteins. We determined the crystal structure of a large section of the protein Cep3 from CBF3, which is the only component with obvious DNA-binding motifs. The protein adopts a roughly bilobal shape, with an extended dimerization interface. The dimer has a large central channel that is sufficient to accommodate duplex B-form DNA. The zinc-finger domains emerge at the edges of the channel, and could bind to the DNA in a pseudo-symmetrical manner at degenerate half-sites in the centromeric sequence. We propose a mechanism for the modulation of DNA affinity by an acidic activator domain, which could be applicable to a wider family of transcription factors.

PubMed: 18064045
DOI: 10.1038/SJ.EMBOR.7401139

実験手法

X-RAY DIFFRACTION (2.49 Å)