2VE9

Xray structure of KOPS bound gamma domain of FtsK (P. aeruginosa)

2VE9 の概要

• エントリーDOI: 10.2210/pdb2ve9/pdb
• 関連するPDBエントリー: 2IUT 2IUU 2J5O 2VE8
• 分子名称: DNA TRANSLOCASE FTSK, 5'-D(*AP*CP*CP*AP*GP*GP*GP*CP*AP*GP *GP*GP*CP*GP*AP*C)-3', 5'-D(*GP*TP*CP*GP*CP*CP*CP*TP*GP*CP *CP*CP*TP*GP*GP*T)-3', ... (5 entities in total)
• 機能のキーワード: nucleotide-binding, chromosome partition, atp-binding, dna-binding, translocase, winged helix, bacterial cell division, transport protein, cell division, transmembrane, inner membrane, ftsz, ftsk, membrane, cell cycle, dna binding
• 由来する生物種: PSEUDOMONAS AERUGINOSA; 詳細
• 細胞内の位置: Cell inner membrane; Multi-pass membrane protein; Cytoplasmic side: Q9I0M3
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 67240.51

構造登録者

Lowe, J.,Allen, M.D.,Sherratt, D.J. (登録日: 2007-10-17, 公開日: 2008-09-09, 最終更新日: 2024-05-08)

主引用文献

Lowe, J.,Ellonen, A.,Allen, M.D.,Atkinson, C.,Sherratt, D.J.,Grainge, I.
Molecular Mechanism of Sequence-Directed DNA Loading and Translocation by Ftsk.
Mol.Cell, 31:498-, 2008

PubMed Abstract: Dimeric circular chromosomes, formed by recombination between monomer sisters, cannot be segregated to daughter cells at cell division. XerCD site-specific recombination at the Escherichia coli dif site converts these dimers to monomers in a reaction that requires the DNA translocase FtsK. Short DNA sequences, KOPS (GGGNAGGG), which are polarized toward dif in the chromosome, direct FtsK translocation. FtsK interacts with KOPS through a C-terminal winged helix domain gamma. The crystal structure of three FtsKgamma domains bound to 8 bp KOPS DNA demonstrates how three gamma domains recognize KOPS. Using covalently linked dimers of FtsK, we infer that three gamma domains per hexamer are sufficient to recognize KOPS and load FtsK and subsequently activate recombination at dif. During translocation, FtsK fails to recognize an inverted KOPS sequence. Therefore, we propose that KOPS act solely as a loading site for FtsK, resulting in a unidirectionally oriented hexameric motor upon DNA.

PubMed: 18722176
DOI: 10.1016/J.MOLCEL.2008.05.027

実験手法

X-RAY DIFFRACTION (1.9 Å)