2VDA

Solution structure of the SecA-signal peptide complex

2VDA の概要

• エントリーDOI: 10.2210/pdb2vda/pdb
• 関連するPDBエントリー: 1M6N 1TF5 1TM6 2FSF
• 分子名称: TRANSLOCASE SUBUNIT SECA, MALTOPORIN (2 entities in total)
• 機能のキーワード: sugar transport, protein transport, protein targeting, transmembrane, outer membrane, signal peptide, paramagnetic relaxation enhancement, translocase, ion transport, translocation, protein secretion, nucleotide-binding, seca, porin, membrane, transport, atp-binding, high molecular weight complex
• 由来する生物種: ESCHERICHIA COLI; 詳細
• 細胞内の位置: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side: P10408; Cell outer membrane ; Multi-pass membrane protein : Q8CVI4
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 96990.49

構造登録者

Gelis, I.,Bonvin, A.M.J.J.,Keramisanou, D.,Koukaki, M.,Gouridis, G.,Karamanou, S.,Economou, A.,Kalodimos, C.G. (登録日: 2007-10-01, 公開日: 2007-11-27, 最終更新日: 2024-05-15)

主引用文献

Gelis, I.,Bonvin, A.M.J.J.,Keramisanou, D.,Koukaki, M.,Gouridis, G.,Karamanou, S.,Economou, A.,Kalodimos, C.G.
Structural Basis for Signal-Sequence Recognition by the Translocase Motor Seca as Determined by NMR
Cell(Cambridge,Mass.), 131:756-, 2007

PubMed Abstract: Recognition of signal sequences by cognate receptors controls the entry of virtually all proteins to export pathways. Despite its importance, this process remains poorly understood. Here, we present the solution structure of a signal peptide bound to SecA, the 204 kDa ATPase motor of the Sec translocase. Upon encounter, the signal peptide forms an alpha-helix that inserts into a flexible and elongated groove in SecA. The mode of binding is bimodal, with both hydrophobic and electrostatic interactions mediating recognition. The same groove is used by SecA to recognize a diverse set of signal sequences. Impairment of the signal-peptide binding to SecA results in significant translocation defects. The C-terminal tail of SecA occludes the groove and inhibits signal-peptide binding, but autoinhibition is relieved by the SecB chaperone. Finally, it is shown that SecA interconverts between two conformations in solution, suggesting a simple mechanism for polypeptide translocation.

PubMed: 18022369
DOI: 10.1016/J.CELL.2007.09.039

実験手法

SOLUTION NMR