2VC8

Crystal structure of the LSm domain of human EDC3 (enhancer of decapping 3)

2VC8 の概要

• エントリーDOI: 10.2210/pdb2vc8/pdb
• 関連するPDBエントリー: 2RM4
• 分子名称: ENHANCER OF MRNA-DECAPPING PROTEIN 3 (2 entities in total)
• 機能のキーワード: p-body component, enhancer of mrna decapping, rna, cytoplasm, sm-like protein, protein-binding, protein binding
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Cytoplasm, P-body: Q96F86
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8988.08

構造登録者

Tritschler, F.,Hartmann, M.D.,Weichenrieder, O. (登録日: 2007-09-19, 公開日: 2007-10-16, 最終更新日: 2024-05-08)

主引用文献

Tritschler, F.,Eulalio, A.,Truffault, V.,Hartmann, M.D.,Helms, S.,Schmidt, S.,Coles, M.,Izaurralde, E.,Weichenrieder, O.
A Divergent Sm Fold in Edc3 Proteins Mediates Dcp1 Binding and P-Body Targeting.
Mol.Cell.Biol., 27:8600-, 2007

PubMed Abstract: Members of the (L)Sm (Sm and Sm-like) protein family are found across all kingdoms of life and play crucial roles in RNA metabolism. The P-body component EDC3 (enhancer of decapping 3) is a divergent member of this family that functions in mRNA decapping. EDC3 is composed of a N-terminal LSm domain, a central FDF domain, and a C-terminal YjeF-N domain. We show that this modular architecture enables EDC3 to interact with multiple components of the decapping machinery, including DCP1, DCP2, and Me31B. The LSm domain mediates DCP1 binding and P-body localization. We determined the three-dimensional structures of the LSm domains of Drosophila melanogaster and human EDC3 and show that the domain adopts a divergent Sm fold that lacks the characteristic N-terminal alpha-helix and has a disrupted beta4-strand. This domain remains monomeric in solution and lacks several features that canonical (L)Sm domains require for binding RNA. The structures also revealed a conserved patch of surface residues that are required for the interaction with DCP1 but not for P-body localization. The conservation of surface and of critical structural residues indicates that LSm domains in EDC3 proteins adopt a similar fold that has separable novel functions that are absent in canonical (L)Sm proteins.

PubMed: 17923697
DOI: 10.1128/MCB.01506-07

実験手法

X-RAY DIFFRACTION (1.31 Å)