2VBT

Riboflavin kinase Mj0056 from Methanocaldococcus jannaschii in complex with CDP and PO4

Summary for 2VBT

• Entry DOI: 10.2210/pdb2vbt/pdb
• Related: 2P3M 2VBS 2VBU 2VBV
• Descriptor: RIBOFLAVIN KINASE, CYTIDINE-5'-DIPHOSPHATE, SODIUM ION, ... (5 entities in total)
• Functional Keywords: transferase, cradle-loop barrel, ctp-dependent kinase, fmn
• Biological source: METHANOCOCCUS JANNASCHII
• Total number of polymer chains: 1
• Total formula weight: 16230.66

Authors

Hartmann, M.D.,Ammelburg, M.,Djuranovic, S.,Martin, J.,Lupas, A.N.,Zeth, K. (deposition date: 2007-09-16, release date: 2007-11-20, Last modification date: 2023-12-13)

Primary citation

Ammelburg, M.,Hartmann, M.D.,Djuranovic, S.,Alva, V.,Koretke, K.K.,Martin, J.,Sauer, G.,Truffault, V.,Zeth, K.,Lupas, A.N.,Coles, M.
A Ctp-Dependent Archaeal Riboflavin Kinase Forms a Bridge in the Evolution of Cradle-Loop Barrels.
Structure, 15:1577-, 2007

PubMed Abstract: Proteins of the cradle-loop barrel metafold are formed by duplication of a conserved betaalphabeta-element, suggesting a common evolutionary origin from an ancestral group of nucleic acid-binding proteins. The basal fold within this metafold, the RIFT barrel, is also found in a wide range of enzymes, whose homologous relationship with the nucleic acid-binding group is unclear. We have characterized a protein family that is intermediate in sequence and structure between the basal group of cradle-loop barrels and one family of RIFT-barrel enzymes, the riboflavin kinases. We report the structure, substrate-binding mode, and catalytic activity for one of these proteins, Methanocaldococcus jannaschii Mj0056, which is an archaeal riboflavin kinase. Mj0056 is unusual in utilizing CTP rather than ATP as the donor nucleotide, and sequence conservation in the relevant residues suggests that this is a general feature of archaeal riboflavin kinases.

PubMed: 18073108
DOI: 10.1016/J.STR.2007.09.027

Experimental method

X-RAY DIFFRACTION (2.7 Å)