2VBM

Tailspike protein of bacteriophage Sf6 complexed with tetrasaccharide

2VBM の概要

• エントリーDOI: 10.2210/pdb2vbm/pdb
• 関連するPDBエントリー: 2VBE 2VBK
• 分子名称: TAILSPIKE-PROTEIN, alpha-L-rhamnopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-L-rhamnopyranose-(1-2)-alpha-L-rhamnopyranose, PHOSPHATE ION, ... (6 entities in total)
• 機能のキーワード: viral adhesion protein, viral protein, hydrolase, tailspike, endorhamnosidase, right-handed parallel beta-helix
• 由来する生物種: ENTEROBACTERIA PHAGE SF6
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 56016.17

構造登録者

Mueller, J.J.,Barbirz, S.,Freiberg, A.,Seckler, R.,Heinemann, U. (登録日: 2007-09-14, 公開日: 2008-04-22, 最終更新日: 2023-12-13)

主引用文献

Muller, J.J.,Barbirz, S.,Heinle, K.,Freiberg, A.,Seckler, R.,Heinemann, U.
An Intersubunit Active Site between Supercoiled Parallel Beta Helices in the Trimeric Tailspike Endorhamnosidase of Shigella Flexneri Phage Sf6.
Structure, 16:766-, 2008

PubMed Abstract: Sf6 belongs to the Podoviridae family of temperate bacteriophages that infect gram-negative bacteria by insertion of their double-stranded DNA. They attach to their hosts specifically via their tailspike proteins. The 1.25 A crystal structure of Shigella phage Sf6 tailspike protein (Sf6 TSP) reveals a conserved architecture with a central, right-handed beta helix. In the trimer of Sf6 TSP, the parallel beta helices form a left-handed, coiled-beta coil with a pitch of 340 A. The C-terminal domain consists of a beta sandwich reminiscent of viral capsid proteins. Further crystallographic and biochemical analyses show a Shigella cell wall O-antigen fragment to bind to an endorhamnosidase active site located between two beta-helix subunits each anchoring one catalytic carboxylate. The functionally and structurally related bacteriophage, P22 TSP, lacks sequence identity with Sf6 TSP and has its active sites on single subunits. Sf6 TSP may serve as an example for the evolution of different host specificities on a similar general architecture.

PubMed: 18462681
DOI: 10.1016/J.STR.2008.01.019

実験手法

X-RAY DIFFRACTION (2 Å)