2VB6

Myosin VI (MD-insert2-CaM, Delta Insert1) Post-rigor state (crystal form 2)

2VB6 の概要

• エントリーDOI: 10.2210/pdb2vb6/pdb
• 関連するPDBエントリー: 2BKH 2BKI 2V26 2VAS
• 分子名称: MYOSIN VI, CALMODULIN, ADENOSINE-5'-DIPHOSPHATE, ... (7 entities in total)
• 機能のキーワード: myosin vi, post-rigor state, mg.adp.befx, calmodulin, cam, molecular motor, motor protein
• 由来する生物種: SUS SCROFA (PIG); 詳細
• 細胞内の位置: Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein (By similarity): Q29122
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 107418.82

構造登録者

Menetrey, J.,Llinas, P.,Cicolari, J.,Squires, G.,Liu, X.,Li, A.,Sweeney, H.L.,Houdusse, A. (登録日: 2007-09-06, 公開日: 2007-12-11, 最終更新日: 2024-05-08)

主引用文献

Menetrey, J.,Llinas, P.,Cicolari, J.,Squires, G.,Liu, X.,Li, A.,Sweeney, H.L.,Houdusse, A.
The Post-Rigor Structure of Myosin Vi and Implications for the Recovery Stroke.
Embo J., 27:244-, 2008

PubMed Abstract: Myosin VI has an unexpectedly large swing of its lever arm (powerstroke) that optimizes its unique reverse direction movement. The basis for this is an unprecedented rearrangement of the subdomain to which the lever arm is attached, referred to as the converter. It is unclear at what point(s) in the myosin VI ATPase cycle rearrangements in the converter occur, and how this would effect lever arm position. We solved the structure of myosin VI with an ATP analogue (ADP.BeF3) bound in its nucleotide-binding pocket. The structure reveals that no rearrangement in the converter occur upon ATP binding. Based on previously solved myosin structures, our structure suggests that no reversal of the powerstroke occurs during detachment of myosin VI from actin. The structure also reveals novel features of the myosin VI motor that may be important in maintaining the converter conformation during detachment from actin, and other features that may promote rapid rearrangements in the structure following actin detachment that enable hydrolysis of ATP.

PubMed: 18046460
DOI: 10.1038/SJ.EMBOJ.7601937

実験手法

X-RAY DIFFRACTION (2.3 Å)