2VAH

Solution structure of a B-DNA hairpin at low pressure.

2VAH の概要

• エントリーDOI: 10.2210/pdb2vah/pdb
• 関連するPDBエントリー: 1DGO 2VAI
• 分子名称: 5'-D(*AP*GP*GP*AP*TP*CP*CP*TP*UP*TP *TP*GP*GP*AP*TP*CP*CP*T)-3' (1 entity in total)
• 機能のキーワード: dna, b-dna hairpin, pressure
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5483.54

構造登録者

Williamson, M.P.,Wilton, D.J.,Ghosh, M.,Chary, K.V.A.,Akasaka, K. (登録日: 2007-08-31, 公開日: 2007-09-11, 最終更新日: 2024-05-15)

主引用文献

Williamson, M.P.,Wilton, D.J.,Ghosh, M.,Chary, K.V.A.,Akasaka, K.
Structural Change in a B-DNA Helix with Hydrostatic Pressure
Nucleic Acids Res., 36:4032-, 2008

PubMed Abstract: Study of the effects of pressure on macromolecular structure improves our understanding of the forces governing structure, provides details on the relevance of cavities and packing in structure, increases our understanding of hydration and provides a basis to understand the biology of high-pressure organisms. A study of DNA, in particular, helps us to understand how pressure can affect gene activity. Here we present the first high-resolution experimental study of B-DNA structure at high pressure, using NMR data acquired at pressures up to 200 MPa (2 kbar). The structure of DNA compresses very little, but is distorted so as to widen the minor groove, and to compress hydrogen bonds, with AT pairs compressing more than GC pairs. The minor groove changes are suggested to lead to a compression of the hydration water in the minor groove.

PubMed: 18515837
DOI: 10.1093/NAR/GKN350

実験手法

SOLUTION NMR