2V7N

Unusual twinning in crystals of the CitS binding antibody Fab fragment f3p4

Summary for 2V7N

• Entry DOI: 10.2210/pdb2v7n/pdb
• Descriptor: IMMUNOGLOBULIN LIGHT CHAIN, IMMUNOGLOBULIN HEAVY CHAIN (3 entities in total)
• Functional Keywords: binding protein, recombinant fab fragment, synthetic antibody library (hucal), phage display, immune system
• Biological source: SYNTHETIC CONSTRUCT; More
• Total number of polymer chains: 8
• Total formula weight: 191700.66

Authors

Frey, D.,Huber, T.,Plueckthun, A.,Gruetter, M.G. (deposition date: 2007-07-31, release date: 2008-06-17, Last modification date: 2024-10-23)

Primary citation

Frey, D.,Huber, T.,Plueckthun, A.,Gruetter, M.G.
Structure of the Recombinant Antibody Fab Fragment F3P4.
Acta Crystallogr.,Sect.D, 64:636-, 2008

PubMed Abstract: The structure of the antibody Fab fragment f3p4, which was selected from a subset of the synthetic HuCAL antibody library to bind the sodium citrate symporter CitS, is described at 1.92 A resolution. Comparison with computational models revealed deviations in a few framework positions and in the binding loops. The crystals belong to space group P2(1)2(1)2 and contain four molecules in the asymmetric unit, with unit-cell parameters a=102.77, b=185.92, c=102.97 A. These particular unit-cell parameters allowed pseudo-merohedral twinning; interestingly, the twinning law relates a twofold screw axis to a twofold axis.

PubMed: 18560151
DOI: 10.1107/S0907444908007282

Experimental method

X-RAY DIFFRACTION (1.92 Å)