2V7N

Unusual twinning in crystals of the CitS binding antibody Fab fragment f3p4

2V7N の概要

• エントリーDOI: 10.2210/pdb2v7n/pdb
• 分子名称: IMMUNOGLOBULIN LIGHT CHAIN, IMMUNOGLOBULIN HEAVY CHAIN (3 entities in total)
• 機能のキーワード: binding protein, recombinant fab fragment, synthetic antibody library (hucal), phage display, immune system
• 由来する生物種: SYNTHETIC CONSTRUCT; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 191700.66

構造登録者

Frey, D.,Huber, T.,Plueckthun, A.,Gruetter, M.G. (登録日: 2007-07-31, 公開日: 2008-06-17, 最終更新日: 2024-10-23)

主引用文献

Frey, D.,Huber, T.,Plueckthun, A.,Gruetter, M.G.
Structure of the Recombinant Antibody Fab Fragment F3P4.
Acta Crystallogr.,Sect.D, 64:636-, 2008

PubMed Abstract: The structure of the antibody Fab fragment f3p4, which was selected from a subset of the synthetic HuCAL antibody library to bind the sodium citrate symporter CitS, is described at 1.92 A resolution. Comparison with computational models revealed deviations in a few framework positions and in the binding loops. The crystals belong to space group P2(1)2(1)2 and contain four molecules in the asymmetric unit, with unit-cell parameters a=102.77, b=185.92, c=102.97 A. These particular unit-cell parameters allowed pseudo-merohedral twinning; interestingly, the twinning law relates a twofold screw axis to a twofold axis.

PubMed: 18560151
DOI: 10.1107/S0907444908007282

実験手法

X-RAY DIFFRACTION (1.92 Å)