2V6L

Molecular Model of a Type III Secretion System Needle

2V6L の概要

• エントリーDOI: 10.2210/pdb2v6l/pdb
• 関連するPDBエントリー: 2CA5
• EMDBエントリー: 1416
• 分子名称: MXIH (1 entity in total)
• 機能のキーワード: t3ss, virulence, transport, protein transport
• 由来する生物種: SHIGELLA FLEXNERI
• タンパク質・核酸の鎖数: 28
• 化学式量合計: 259623.42

構造登録者

Deane, J.E.,Roversi, P.,Cordes, F.S.,Johnson, S.,Kenjale, R.,Daniell, S.,Booy, F.,Picking, W.L.,Picking, W.D.,Blocker, A.J.,Lea, S.M. (登録日: 2007-07-19, 公開日: 2007-07-31, 最終更新日: 2024-05-08)

主引用文献

Deane, J.E.,Roversi, P.,Cordes, F.S.,Johnson, S.,Kenjale, R.,Daniell, S.,Booy, F.,Picking, W.D.,Picking, W.L.,Blocker, A.J.,Lea, S.M.
Molecular model of a type III secretion system needle: Implications for host-cell sensing.
Proc. Natl. Acad. Sci. U.S.A., 103:12529-12533, 2006

PubMed Abstract: Type III secretion systems are essential virulence determinants for many Gram-negative bacterial pathogens. The type III secretion system consists of cytoplasmic, transmembrane, and extracellular domains. The extracellular domain is a hollow needle protruding above the bacterial surface and is held within a basal body that traverses both bacterial membranes. Effector proteins are translocated, via this external needle, directly into host cells, where they subvert normal cell functions to aid infection. Physical contact with host cells initiates secretion and leads to formation of a pore, thought to be contiguous with the needle channel, in the host-cell membrane. Here, we report the crystal structure of the Shigella flexneri needle subunit MxiH and a complete model for the needle assembly built into our three-dimensional EM reconstruction. The model, combined with mutagenesis data, reveals that signaling of host-cell contact is relayed through the needle via intersubunit contacts and suggests a mode of binding for a tip complex.

PubMed: 16888041
DOI: 10.1073/pnas.0602689103

実験手法

ELECTRON MICROSCOPY (16 Å)