2V2K

THE CRYSTAL STRUCTURE OF FDXA, A 7FE FERREDOXIN FROM MYCOBACTERIUM SMEGMATIS

2V2K の概要

• エントリーDOI: 10.2210/pdb2v2k/pdb
• 分子名称: FERREDOXIN, FE3-S4 CLUSTER, ACETATE ION, ... (4 entities in total)
• 機能のキーワード: iron, transport, iron-sulfur, mycobacterium tuberculosis, fe-s cluster, metal-binding, 7fe ferredoxin, electron transfer, electron transport, mycobacterium smegmatis, 4fe-4s cluster instability
• 由来する生物種: MYCOBACTERIUM SMEGMATIS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 24107.42

構造登録者

Ricagno, S.,de Rosa, M.,Aliverti, A.,Zanetti, G.,Bolognesi, M. (登録日: 2007-06-06, 公開日: 2007-07-03, 最終更新日: 2024-11-13)

主引用文献

Ricagno, S.,De Rosa, M.,Aliverti, A.,Zanetti, G.,Bolognesi, M.
The Crystal Structure of Fdxa, a 7Fe Ferredoxin from Mycobacterium Smegmatis.
Biochem.Biophys.Res.Commun., 360:97-, 2007

PubMed Abstract: Mycobacterium smegmatis ferredoxin FdxA, which has an orthologue ferredoxin in Mycobacterium tuberculosis, FdxC, contains both one [3Fe-4S] and one [4Fe-4S] cluster. M. smegmatis FdxA has been shown to be a preferred ferredoxin substrate of FprA [F. Fischer, D. Raimondi, A. Aliverti, G. Zanetti, Mycobacterium tuberculosis FprA, a novel bacterial NADPH-ferredoxin reductase, Eur. J. Biochem. 269 (2002) 3005-3013], an adrenodoxin reductase-like flavoprotein of M. tuberculosis, suggesting that M. tuberculosis FdxC could be the physiological partner of the enzyme in providing reducing power to the cytochromes P450. We report here the crystal structure of FdxA at 1.6A resolution (R(factor) 16.5%, R(free) 20.2%). Besides providing an insight on protein architecture for this 106-residue ferredoxin, our crystallographic investigation highlights lability of the [4Fe-4S] center, which is shown to loose a Fe atom during crystal growth. Due to their high similarity (87% sequence identity), the structure here reported can be considered a valuable model for M. tuberculosis FdxC, thus representing a step forward in the study of the complex mycobacterial redox pathways.

PubMed: 17577575
DOI: 10.1016/J.BBRC.2007.06.013

実験手法

X-RAY DIFFRACTION (1.6 Å)