2V26

Myosin VI (MD) pre-powerstroke state (Mg.ADP.VO4)

2V26 の概要

• エントリーDOI: 10.2210/pdb2v26/pdb
• 関連するPDBエントリー: 2BKH 2BKI
• 分子名称: MYOSIN VI, ADENOSINE-5'-DIPHOSPHATE, VANADATE ION, ... (7 entities in total)
• 機能のキーワード: calmodulin-binding, nucleotide-binding, myosin, membrane, vanadate, myosin vi, transport, pre- powerstroke, transition state, protein transport, actin-binding, motor protein, nuclear protein, endocytosis, atp-binding, coiled coil, domain motor, golgi apparatus, phosphorylation, molecular motor, structural protein
• 由来する生物種: SUS SCROFA (PIG)
• 細胞内の位置: Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein (By similarity): Q29122
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 90625.80

構造登録者

Menetrey, J.,Llinas, P.,Mukherjea, M.,Sweeney, H.L.,Houdusse, A. (登録日: 2007-06-03, 公開日: 2007-11-20, 最終更新日: 2023-12-13)

主引用文献

Menetrey, J.,Llinas, P.,Mukherjea, M.,Sweeney, H.L.,Houdusse, A.
The Structural Basis for the Large Powerstroke of Myosin Vi.
Cell(Cambridge,Mass.), 131:300-, 2007

PubMed Abstract: Due to a unique addition to the lever arm-positioning region (converter), class VI myosins move in the opposite direction (toward the minus-end of actin filaments) compared to other characterized myosin classes. However, the large size of the myosin VI lever arm swing (powerstroke) cannot be explained by our current view of the structural transitions that occur within the myosin motor. We have solved the crystal structure of a fragment of the myosin VI motor in the structural state that represents the starting point for movement on actin; the pre-powerstroke state. Unexpectedly, the converter itself rearranges to achieve a conformation that has not been seen for other myosins. This results in a much larger powerstroke than is achievable without the converter rearrangement. Moreover, it provides a new mechanism that could be exploited to increase the powerstroke of yet to be characterized plus-end-directed myosin classes.

PubMed: 17956731
DOI: 10.1016/J.CELL.2007.08.027

実験手法

X-RAY DIFFRACTION (1.75 Å)