2UY1

CRYSTAL STRUCTURE OF CSTF-77

2UY1 の概要

• エントリーDOI: 10.2210/pdb2uy1/pdb
• 分子名称: CLEAVAGE STIMULATION FACTOR 77 (3 entities in total)
• 機能のキーワード: rna-binding protein, rna binding protein
• 由来する生物種: ENCEPHALITOZOON CUNICULI; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 116450.78

構造登録者

Legrand, P.,Pinaud, N.,Minvielle-Sebastia, L.,Fribourg, S. (登録日: 2007-04-02, 公開日: 2007-07-03, 最終更新日: 2024-05-08)

主引用文献

Legrand, P.,Pinaud, N.,Minvielle-Sebastia, L.,Fribourg, S.
The Structure of the Cstf-77 Homodimer Provides Insights Into Cstf Assembly.
Nucleic Acids Res., 35:4515-, 2007

PubMed Abstract: The cleavage stimulation factor (CstF) is essential for the first step of poly(A) tail formation at the 3' ends of mRNAs. This heterotrimeric complex is built around the 77-kDa protein bridging both CstF-64 and CstF-50 subunits. We have solved the crystal structure of the 77-kDa protein from Encephalitozoon cuniculi at a resolution of 2 A. The structure folds around 11 Half-a-TPR repeats defining two domains. The crystal structure reveals a tight homodimer exposing phylogenetically conserved areas for interaction with protein partners. Mapping experiments identify the C-terminal region of Rna14p, the yeast counterpart of CstF-77, as the docking domain for Rna15p, the yeast CstF-64 homologue.

PubMed: 17584787
DOI: 10.1093/NAR/GKM458

実験手法

X-RAY DIFFRACTION (2 Å)