2UX2

High resolution structure of human CD59

2UX2 の概要

• エントリーDOI: 10.2210/pdb2ux2/pdb
• 関連するPDBエントリー: 1CDQ 1CDR 1CDS 1ERG 1ERH 2J8B 2UWR
• 分子名称: CD59 GLYCOPROTEIN (2 entities in total)
• 機能のキーワード: mac, mirl, cd59, membrane, gpi-anchor, lipoprotein, glycoprotein, complement regulator, immune system
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Cell membrane; Lipid-anchor, GPI-anchor: P13987
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 27303.91

構造登録者

Leath, K.J.,Johnson, S.,Roversi, P.,Morgan, B.P.,Smith, R.A.G.,Lea, S.M. (登録日: 2007-03-26, 公開日: 2007-08-07, 最終更新日: 2024-11-13)

主引用文献

Leath, K.J.,Johnson, S.,Roversi, P.,Hughes, T.R.,Smith, R.A.G.,Mackenzie, L.,Morgan, B.P.,Lea, S.M.
High-Resolution Structures of Bacterially Expressed Soluble Human Cd59.
Acta Crystallogr.,Sect.F, 63:648-, 2007

PubMed Abstract: CD59 is a membrane-bound glycoprotein that protects host cells from lysis by inhibiting the terminal pathway of complement, preventing the formation and insertion of the membrane attack complex (MAC). Crystals of bacterially expressed and nonglycosylated recombinant soluble human CD59 have been obtained from three crystallization conditions, each of which gave rise to a distinct crystal form. Each crystal form led to a crystal structure at high resolution (1.15, 1.35 and 1.8 A). In one of these structures the electron-density map shows an as yet unidentified small molecule in the predicted C8/C9-binding site. The presence/absence of this ligand is linked to alternate conformations of the amino acids implicated in C8/C9 binding.

PubMed: 17671359
DOI: 10.1107/S1744309107033477

実験手法

X-RAY DIFFRACTION (1.8 Å)